{"product_id":"cst-97099s","title":"CST,  97099S, HEXIM1 (D5Y5K) Rabbit Monoclonal Antibody (PE Conjugate)","description":"Monoclonal Antibody for studying HEXIM1. Validated for Flow Cytometry (Fixed\/Permeabilized). Highly specific and rigorously validated in-house, HEXIM1 (D5Y5K) Rabbit Monoclonal Antibody (PE Conjugate) (CST #97099) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFlow Cytometry (Fixed\/Permeabilized): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg\/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nHEXIM1 (D5Y5K) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total HEXIM1 protein.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly63 of human HEXIM1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nHexamethylene bis-acetamide-inducible protein 1 (HEXIM1) was originally identified in vascular smooth muscle cells as a protein that is upregulated upon treatment with the differentiating agent hexamethylene bisacetamide (1). HEXIM1 binds 7SK RNA, a highly abundant non-coding RNA, and together they act as a potent inhibitor of positive transcription elongation factor b (P-TEFb) (2,3). P-TEFb phosphorylates the C-terminal domain of the largest subunit of RNA polymerase II and is an important regulator of transcription elongation (4-8). 7SK RNA-bound HEXIM1 interacts with the cyclin T1 subunit of P-TEFb, sequestering P-TEFb in an inactive form leading to transcription inhibition (2,3). The regulation of the relative ratio of inactive to active P-TEFb in the cell by HEXIM1\/7SK RNA is thought to play a critical role in regulation of a wide range of cellular gene expression programs such as estrogen and glucocorticoid receptor regulated genes (9-12).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCardiac lineage protein 1; CLP1; EDG1; Estrogen down-regulated gene 1 protein; FLJ13562; hexamethylene bis-acetamide inducible 1; Hexamethylene bis-acetamide-inducible protein 1; hexamethylene bisacetamide inducible 1; hexamethylene bisacetamide-inducible protein; hexamethylene-bis-acetamide-inducible transcript 1; hexamthylene bis-acetamide inducible 1; HEXI1; HEXIM P-TEFb complex subunit 1; HEXIM1; HIS1; HMBA-inducible; MAQ1; menage a quatre 1; Menage a quatre protein 1; Protein HEXIM1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800566452393,"sku":"97099S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-97099s","provider":"Iright","version":"1.0","type":"link"}