{"product_id":"cst-97135t","title":"CST,  97135T, T-bet\/TBX21 (E4I2K) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying TBX21 mouse. Validated for WB,IP,IHC,IF,F,ChIP,C\u0026amp;R. Available in 2 sizes. Highly specific and rigorously validated in-house, T-bet\/TBX21 (E4I2K) Rabbit Monoclonal Antibody (CST #97135) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT\u0026amp;RUN dilution was determined using CUT\u0026amp;RUN Assay Kit # 86652 .\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:200\nImmunohistochemistry (Paraffin): 1:100 - 1:400\nImmunofluorescence (Frozen): 1:400 - 1:1600\nFlow Cytometry (Fixed\/Permeabilized): 1:100 - 1:400\nChromatin IP: 1:50\nCUT\u0026amp;RUN: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 53753 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Flow Cytometry (Fixed\/Permeabilized), Chromatin IP, CUT\u0026amp;RUN\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nT-bet\/TBX21 (E4I2K) Rabbit Monoclonal Antibody recognizes endogenous levels of total T-bet\/TBX21 protein. E412K non-specifically labels the submucosa and external muscularis of mouse colon by immunofluorescence.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a recombinant protein fragment derived from mouse T-bet\/TBX21 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe gene family consists of transcription factors characterized by a related DNA-binding domain (T-box) of approximately 200 amino acids (1,2). The genes exhibit diverse temporal and spatial patterns in the developing embryo. Studies have demonstrated members of this family play crucial roles during embryogenesis in a wide range of organisms by regulating cell fate decisions to establish the early body plan and to regulate later processes underlying organogenesis (3-5). Mutations in genes are associated with many developmental defects (6). Recent studies also indicate potential roles in cancer by members of the T-box family (7-9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nOTTMUSP00000002150; T-bet; T-box 21; T-box protein 21; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; Tbet; Tbly; Tblym; TBT1; Tbx21; Transcription factor TBLYM\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 62\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800060711081,"sku":"97135T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-97135t","provider":"Iright","version":"1.0","type":"link"}