{"product_id":"cst-97728t","title":"CST,  97728T, PBK\/TOPK (F2X2Z) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PBK\/TOPK. Validated for Western Blotting,Simple Western™,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, PBK\/TOPK (F2X2Z) Rabbit Monoclonal Antibody (CST #97728) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunohistochemistry (Paraffin): 1:3200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPBK\/TOPK (F2X2Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total PBK\/TOPK protein. Non-specific cytoplasmic staining was observed in human and mouse skeletal muscle and in mouse spleen by immunohistochemistry.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PBK\/TOPK protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nPBK\/TOPK is a serine\/threonine kinase that is phosphorylated and active during mitosis (1). PBK\/TOPK is composed of kinase subdomains and a carboxy-terminal PDZ-binding domain, which is thought to interact with the human homologue of the Drosophila Discs large (hDlg) tumor suppressor protein (1). Increased PBK\/TOPK expression has been observed in highly proliferative malignant cell lines, and PBK\/TOPK expression is strongly downregulated during terminal differentiation of HL-60 leukemic cells (2,3). PMA-induced kinase activity toward PBK\/TOPK has been observed (4), and cdc2\/cyclin B has been shown to phosphorylate PBK\/TOPK , presumably at Thr9 (5). Potential substrates of PBK\/TOPK include p38 MAPK and c-Myc (3,4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCancer\/testis antigen 84; CT84; epididymis luminal protein 164; FLJ14385; HEL164; Lymphokine-activated killer T-cell-originated protein kinase; MAPKK-like protein kinase; Nori-3; PBK; PDZ binding kinase; PDZ-binding kinase; serine\/threonine protein kinase; Spermatogenesis-related protein kinase; SPK; T-LAK cell-originated protein kinase; TOPK\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 40\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800066642089,"sku":"97728T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-97728t","provider":"Iright","version":"1.0","type":"link"}