{"product_id":"cst-9777s","title":"CST,  9777S, Pirin (1E8) Rat Monoclonal Antibody","description":"Monoclonal Antibody for studying Pirin. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Pirin (1E8) Rat Monoclonal Antibody (CST #9777) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPirin (1E8) Rat Monoclonal Antibody detects endogenous levels of Pirin protein. This antibody does not cross-react with other proteins in the cupin superfamily.\nSpecies Reactivity: Human, Mouse, Rat, Hamster, Monkey, Bovine\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with the full-length Pirin protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nPirin is a highly conserved nuclear protein and a member of the cupin superfamily of proteins, all of which contain two conserved β-barrel fold domains (1). Pirin functions as a co-factor for NFI\/CTF1 and Bcl-3, implicating it in DNA replication, transcriptional activation and apoptosis (2,3). Both human and bacterial pirins catalyze the di-oxygenation of quercetin, one of a class of widespread naturally occurring flavenoid compounds that have anti-inflammatory and anti-cancer activities (4). Flavenoids exert these beneficial activities by functioning as antioxidants that stabilize cellular free radical molecules and by directly modulating cell signaling pathways involving PI 3-kinase, Akt\/PKB, PKC and MAP kinases (5). Quercetin has also been directly implicated in the regulation of NF-κB activity; thus, Pirin may exert its apoptotic functions both by directly regulating Bcl-3\/NF-κB activity and by modulating quercetin levels in the cell (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nPIR; Pirin; pirin (iron-binding nuclear protein); Probable quercetin 2,3-dioxygenase PIR; Probable quercetinase\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Hm Mk B\nSENSITIVITY: Endogenous\nMW (kDa): 32\nSource\/Isotype: Rat IgG2a","brand":"CST","offers":[{"title":"Default Title","offer_id":46800066904233,"sku":"9777S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9777s","provider":"Iright","version":"1.0","type":"link"}