{"product_id":"cst-9814t","title":"CST,  9814T, Acetylated-Lysine (Ac-K2-100) MultiMab® Rabbit Monoclonal Antibody mix","description":"Monoclonal Antibody for studying . Validated for WB,IP,ChIP,E. Available in 2 sizes. Highly specific and rigorously validated in-house, Acetylated-Lysine (Ac-K\u003csup\u003e2\u003c\/sup\u003e-100) MultiMab\u003csup\u003e®\u003c\/sup\u003e Rabbit Monoclonal Antibody mix (CST #9814) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nChromatin IP: 1:50\nPeptide ELISA (DELFIA): 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nAcetylated-Lysine (Ac-K\nSpecies Reactivity: All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMultiMab rabbit monoclonal mix antibodies are prepared by combining individual rabbit monoclonal clones in optimized ratios for the approved applications. Each antibody in the mix is carefully selected based on motif recognition and performance in multiple assays. Each mix is engineered to yield the broadest possible coverage of the modification being studied while ensuring a high degree of specificity for the modification or motif.\n\u003cb\u003eBackground\u003c\/b\u003e\nAcetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (1). Signaling resulting in acetylation\/deacetylation of histones, transcription factors, and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation, and apoptosis (2-6). Recent proteomic surveys suggest that acetylation of lysine residues may be a widespread and important form of post-translational protein modification that affects thousands of proteins involved in control of cell cycle and metabolism, longevity, actin polymerization, and nuclear transport (7,8). The regulation of protein acetylation status is impaired in cancer and polyglutamine diseases (9), and HDACs have become promising targets for anti-cancer drugs currently in development (10).\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800239853737,"sku":"9814T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9814t","provider":"Iright","version":"1.0","type":"link"}