{"product_id":"cst-98439sf","title":"CST,  98439SF, LC3A\/B (D7H3T) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying LC3B. Validated for ELISA+. Highly specific and rigorously validated in-house, LC3A\/B (D7H3T) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #98439) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free.\u0026lt;\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nLC3A\/B (D7H3T) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous\/transfected levels of LC3A\/B.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with synthetic peptide corresponding to residues surrounding Pro45 of human LC3B protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nAutophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation, but it has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection, and cancer (3). Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4) and subsequently found to contain similarity to the yeast protein Apg8\/Aut7\/Cvt5 critical for autophagy (5). Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo posttranslational modifications during autophagy (6-9). Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form. During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10). The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form, LC3-II, have been used as indicators of autophagy (11).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nATG8F; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; LC3B; MAP1 light chain 3-like protein 2; MAP1A\/1BLC3; MAP1A\/MAP1B LC3 B; MAP1A\/MAP1B light chain 3 B; MAP1ALC3; MAP1LC3B; MAP1LC3B-a; microtubule associated protein 1 light chain 3 beta; Microtubule-associated protein 1 light chain 3 beta; microtubule-associated proteins 1A\/1B light chain 3; Microtubule-associated proteins 1A\/1B light chain 3B; MLP3B\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800327475369,"sku":"98439SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-98439sf","provider":"Iright","version":"1.0","type":"link"}