{"product_id":"cst-98611s","title":"CST,  98611S, CRACC\/SLAMF7\/CD319 (E5C4M) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CRACC\/SLAMF7. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CRACC\/SLAMF7\/CD319 (E5C4M) Rabbit Monoclonal Antibody (CST #98611) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nIHC Leica Bond: 1:400\nImmunohistochemistry (Paraffin): 1:150\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 66110 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCRACC\/SLAMF7\/CD319 (E5C4M) Rabbit Monoclonal Antibody recognizes endogenous levels of total CRACC\/SLAMF7\/CD319 protein. This antibody may cross-react with several isoforms of CRACC\/SLAMF7\/CD319 and with varying levels of glycosylation. Non-specific staining was observed in prostate epithelium.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CRACC\/SLAMF7\/CD319 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCRACC\/SLAMF7\/CD319 (also known as CS1) is a member of the signaling lymphocytic activation molecule (SLAM) family. It is a single-pass type l transmembrane glycoprotein expressed on NK cells, subsets of mature dendritic cells, activated B and T lymphocytes, but not in promyelocytic B or T cell lines. Expression of this protein has been detected in the spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung, and trachea (1-5). Homophilic interactions of CRACC\/SLAMF7\/CD319 modulate the activity and differentiation of immune cells. CRACC\/SLAMF7\/CD319 may function as an inhibitory or activating receptor in immune cells depending on cellular context and availability of adapter proteins, SH2D1A\/SAP and\/or SH2D1B\/EAT-2 (4-8). In the presence of SH2D1B\/EAT-2, CRACC\/SLAMF7\/CD319 activates NK cells and B cells (4-6). T cells lack SH2D1B\/EAT-2 expression, and therefore CRACC\/SLAMF7\/CD319 acts as an inhibitory receptor (7). In LPS-activated monocytes, CRACC\/SLAMF7\/CD319 negatively regulates production of proinflammatory cytokines (8). CRACC\/SLAMF7\/CD319 is upregulated in multiple myeloma and is implicated in the uncontrolled proliferation of these cells, and thus has become the target for therapeutic intervention (9,10). Seven isoforms of CRACC\/SLAMF7\/CD319 produced by alternative splicing have been identified.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n19A; 19A24 protein; CD2 subset 1; CD2-like receptor activating cytotoxic cells; CD2-like receptor-activating cytotoxic cells; CD319; CRACC; CS1; Membrane protein FOAP-12; Novel Ly9; novel LY9 (lymphocyte antigen 9) like protein; Protein 19A; SLAF7; SLAM family member 7; SLAMF7\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 25-80\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46801037656233,"sku":"98611S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-98611s","provider":"Iright","version":"1.0","type":"link"}