{"product_id":"cst-98778s","title":"CST,  98778S, TMEM119 (E4B9S) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying TMEM119 mouse. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, TMEM119 (E4B9S) Mouse Monoclonal Antibody (CST #98778) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nImmunofluorescence (Frozen): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunofluorescence (Frozen)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTMEM119 (E4B9S) Mouse Monoclonal Antibody recognizes endogenous levels of total TMEM119 protein by IF-Frozen but is not sensitive enough to detect endogenous levels by IF-IC.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of mouse TMEM119 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nTransmembrane protein 119 (TMEM119) is a cell-surface protein of unknown function, expressed exclusively by the microglia subset of myeloid and neural cells (1). Iba1+ microglia with both ramified and amoeboid morphologies express TMEM119, while Iba1+ macrophages are TMEM119 negative (2). and other homeostatic genes have been shown to be downregulated in disease-associated microglia (DAM). DAM are conserved in human and mouse and have been identified in Alzheimer's disease (AD), amyotrophic lateral sclerosis (ALS), and 5XFAD and APP\/PS1 mouse models (3,4). This protein's specificity as a microglia marker has proven itself important for labeling microglia in healthy tissue as well as deciphering them from infiltrating macrophages and other cell types in neurodegenerative disease models (1,2).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAW208946; BC025600; MGC38046; obif; Osteoblast induction factor; TM119; Tmem119; Transmembrane protein 119\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nSource\/Isotype: Mouse IgG1 kappa","brand":"CST","offers":[{"title":"Default Title","offer_id":46800303030441,"sku":"98778S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-98778s","provider":"Iright","version":"1.0","type":"link"}