{"product_id":"cst-99106t","title":"CST,  99106T, Eosinophil cationic protein (F3G7N) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying RNASE3 mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Eosinophil cationic protein (F3G7N) Rabbit Monoclonal Antibody (CST #99106) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunohistochemistry (Paraffin): 1:100 - 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 77698 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nEosinophil cationic protein (F3G7N) Rabbit Monoclonal Antibody recognizes endogenous levels of total Eosinophil cationic protein. By overexpression, this antibody cross-reacts with mouse Eosinophil cationic-type ribonuclease 3\/Ear3 protein but not Eosinophil cationic protein 2\/Ear2, Eosinophil-associated ribonuclease 10\/Ear10, or Eosinophil cationic-type ribonuclease 5\/Ear5 proteins. This antibody detects bands of unknown origin at 50 and 70 kDa in some cell lines and tissues. Non-specific staining was observed in crypts of mouse stomach and small intestine by immunohistochemistry.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro30 of mouse Eosinophil cationic protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nEosinophil cationic protein (ECP, RNASE3) is a member of the ribonuclease superfamily which has both cytotoxic and noncytotoxic properties (1-4). ECP is primarily produced and stored in the secondary granules of eosinophils, where it is involved in host defense against parasites, bacteria, and viruses (1-4). It has also been detected in the primary granules of circulating neutrophils, and can be synthesized by stimulated neutrophils (3,4). ECP is often used as a marker for eosinophilic disease and is measurable in samples such as serum, bronchoalveolar lavage, and nasal secretions (4). A large number of clinical studies are focused on the role of ECP in eosinophil-related disorders, particularly in asthma (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nEar-1; Ear1; ECP 1; ECP1; Eosinophil cationic protein 1; Eosinophil secondary granule ribonuclease 1; eosinophil-associated ribonuclease 1; eosinophil-associated, ribonuclease A family, member 1; Raf1; Ribonuclease 3-1; ribonuclease A f1; RNase 3-1; RNASE3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 18\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800178217129,"sku":"99106T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-99106t","provider":"Iright","version":"1.0","type":"link"}