{"product_id":"cst-99633t","title":"CST,  99633T, HOIP\/RNF31 (E6M5B) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying HOIP. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, HOIP\/RNF31 (E6M5B) Rabbit Monoclonal Antibody (CST #99633) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nHOIP\/RNF31 (E6M5B) Rabbit Monoclonal Antibody recognizes endogenous levels of total HOIP\/RNF31 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human HOIP\/RNF31 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nHOIL-1-interacting protein (HOIP\/RNF31), a RING-type E3 ubiquitin ligase, is the catalytic subunit of the Linear Ubiquitin Chain Assembly Complex (LUBAC) that is associated with TNF-R1 (1). Research studies have shown that the LUBAC consists of three subunits: HOIP, HOIL-1L, and Sharpin that facilitate canonical NF-kB activation in response to pro inflammatory cytokines through M1-linked linear ubiquitination of NEMO and RIP1 (2-6). As part of the LUBAC, HOIP has also been implicated in the negative regulation of interferon-mediated antiviral signaling through the suppression of RIG-I activation (7). The role of HOIP in LUBAC function and human disease is underscored by naturally occurring mutations in HOIP that impair LUBAC assembly and NF-kb activation. Patients that are homozygous for this mutation in HOIP have multi organ auto inflammation and immunodeficiency (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nE3 ubiquitin-protein ligase RNF31; FLJ10111; FLJ23501; HOIL-1-interacting protein; HOIP; MGC19975; Paul; RING finger protein 31; RING-type E3 ubiquitin transferase RNF31; RNF31; ZIBRA; Zinc in-between-RING-finger ubiquitin-associated domain protein\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 120\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46801042866345,"sku":"99633T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-99633t","provider":"Iright","version":"1.0","type":"link"}