{"product_id":"cst-9995s","title":"CST,  9995S, Ionomycin, Calcium Salt","description":"Chemical Modulators for studying in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nIonomycin, Calcium Salt is supplied as 2.5 mg powder. Store at or below -20ºC. Before use, dissolve powder in 1.1 ml DMSO to make a 3 mM ionomycin stock solution. For working concentrations of 1 μM-3 μM, dilute DMSO stock 1:3000 to 1:1000. Treat cells with the desired concentration for intended time.\n\u003cb\u003eStorage\u003c\/b\u003e\nStore lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze\/thaw cycles.\n\u003cb\u003eBackground\u003c\/b\u003e\nIonomycin is a potent and selective calcium ionophore agent (1,2). The molecule acts as a motile Ca carrier and enhances Ca influx by direct stimulation of store-regulated cation entry across biological membranes (3). At the micromolar level, ionomycin can activate Ca \/Calmodulin dependent kinase and phosphatase to stimulate gene expression (4). In human T cells, ionomycin induces hydrolysis of phosphoinositides and activates PKC to mediate T cell activation (5). Ionomycin treatment of human B cells induces the activation of calcium-dependent endonuclease and results in apoptosis (6). Ionomycin treatment of bovine aortic endothelial cells (BAECs) induces rapid dephosphorylation of eNOS at Thr495 and mediates eNOS activation (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBAECs; bovine aortic endothelial cells; calcium carrier; calcium ionphore agent; calmodulin; eNOS; PKC","brand":"CST","offers":[{"title":"Default Title","offer_id":46800702046377,"sku":"9995S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-9995s","provider":"Iright","version":"1.0","type":"link"}