{"product_id":"cst-99966t","title":"CST,  99966T, Mesothelin (D9R5G) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Mesothelin. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Mesothelin (D9R5G) Rabbit Monoclonal Antibody (CST #99966) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunohistochemistry (Paraffin): 1:250\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 50126 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nMesothelin (D9R5G) Rabbit Monoclonal Antibody recognizes endogenous levels of total mesothelin protein. This antibody detects both uncleaved (full-length) and cleaved forms of mesothelin, but does not detect the cleaved fragment corresponding to megakaryocyte-potentiating factor (MPF).\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to human mesothelin protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe gene encodes a 69 kDa precursor protein that is proteolytically cleaved to yield megakaryocyte potentiating factor (MPF) and a GPI-anchored membrane protein termed mesothelin (1). Expression of (cleaved) mesothelin is largely confined to mesothelial cells of normal pleura, pericardium, and peritoneum, but has been reported to be overexpressed in some cancers, including mesothelioma, and some pancreatic and ovarian adenocarcinomas (1,2). Although suggested to be involved in cell adhesion, the physiological functions of mesothelin have not been determined. It is known, however, that mesothelin can be shed from the cell surface following cleavage by TNF-α converting enzyme. Research studies show that serum levels of mesothelin are markedly increased in patients with mesothelioma and ovarian cancer (1), suggesting that serum mesothelin levels may have utility as a cancer biomarker (1-3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCAK1; CAK1 antigen; megakaryocyte potentiating factor; Megakaryocyte-potentiating factor; Mesothelin; mesothelin isoform 1 preproprotein; mesothelin isoform 2 preproprotein; Mesothelin, cleaved form; MPF; MSLN; Pre-pro-megakaryocyte-potentiating factor; Q13421; Q13421-2; Q13421-3; Q13421-4; SMR; SMRP; soluble MPF mesothelin related protein\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 46-48, 70\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46801044340905,"sku":"99966T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-99966t","provider":"Iright","version":"1.0","type":"link"}