{"product_id":"neb-m0206s","title":"New England Biolabs, M0206S, Exonuclease III (E. coli)","description":"\u003cb\u003eRelated Categories\u003c\/b\u003e Exonucleases and Non-specific Endonucleases  \u003cb\u003eApplications\u003c\/b\u003e Polymerases for DNA Manipulation  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eUnit Definition\u003c\/b\u003e One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble total nucleotide in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X NEBuffer 1 with 0.15 mM sonicated duplex [ 3 H]-DNA.  \u003cb\u003eReaction Conditions\u003c\/b\u003e 1X NEBuffer™ 1 Incubate at 37°C 1X NEBuffer™ 1 10 mM Bis-Tris-Propane-HCl 10 mM MgCl2 1 mM DTT (pH 7 @ 25°C)  \u003cb\u003eStorage Buffer\u003c\/b\u003e 5 mM KPO4 200 mM KCl 5 mM β-ME 0.05 mM EDTA 200 µg\/ml BSA 50% Glycerol pH 6.5 @ 25°C  \u003cb\u003eHeat Inactivation\u003c\/b\u003e 70°C for 20 minutes  \u003cb\u003eSpecific Activity\u003c\/b\u003e 150,000 units\/mg  \u003cb\u003eFAQ\u003c\/b\u003e Q: What is the activity of M0206 Exonuclease III in various NEB buffers? A: The activity of Exonuclease III in various buffers is listed below: NEBuffer 1 - 100% NEBuffer 2 - 75% NEBuffer 3 -25% NEBuffer 4 - 75% CutSmart – 100% Q: How does T5 Exonuclease differ from Exonuclease III (NEB# M0206)? A: Exonuclease III acts in the 3' to 5' direction, opposite that of T5 Exonuclease, which acts in the 5’ to 3’ direction. Q: How Does T7 Exonuclease differ from Exonuclease III (NEB# M0206)? A: Exonuclease III acts in the 3' to 5' direction, opposite that of T7 Exonuclease and Lambda Exonuclease. Q: Will Exonuclease III work in rCutSmart buffer? A: Exonuclease III will work in rCutSmart Buffer. To see its % functional activity in rCutSmart, and that of other DNA modifying enzymes in the cloning workflow, refer to the Activity of DNA Modifying Enzymes in rCutSmart® Buffer chart. Q: Can Exonuclease I be used with a double stranded exonuclease to clean up plasmid preparations? A: Exonuclease I can be used with Lambda Exonuclease (NEB# M0262) to clean up plasmid preps. Exonuclease III (NEB# M0206) and T7 Exonuclease (NEB# M0263) will also work, but will damage nicked plasmids. Although Exonuclease I can be used, we recommend using Exonuclease V (RecBCD) (NEB #M0345) to remove chromosomal DNA after plasmid prep (see our Application Note: Using Exonuclease V (RecBCD) to Eliminate Residual Genomic DNA When Purifying Low Copy Plasmids with the Monarch® Plasmid Miniprep Kit. ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835524993193,"sku":"M0206S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-m0206s","provider":"Iright","version":"1.0","type":"link"}