{"product_id":"neb-m0243l","title":"New England Biolabs, M0243L, RNase If","description":"RNase I  \u003cb\u003eRelated Categories\u003c\/b\u003e RNases,, Epitranscriptome Analysis  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eUnit Definition\u003c\/b\u003e One unit is defined as the amount of enzyme required to fully digest 1 picomole of synthetic ssRNA 33-mer in a total reaction volume of 10 μl in 15 minutes in 1X NEBuffer 3 as visualized on a 20% acrylamide gel.  \u003cb\u003eReaction Conditions\u003c\/b\u003e 1X NEBuffer™ 3 Incubate at 37°C 1X NEBuffer™ 3 100 mM NaCl 50 mM Tris-HCl 10 mM MgCl2 1 mM DTT (pH 7.9 @ 25°C)  \u003cb\u003eStorage Buffer\u003c\/b\u003e 10 mM Tris-HCl 100 mM NaCl 1 mM DTT 0.5 mM EDTA 50% Glycerol pH 8 @ 25°C  \u003cb\u003eHeat Inactivation\u003c\/b\u003e 70°C for 20 minutes ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835495600297,"sku":"M0243L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-m0243l","provider":"Iright","version":"1.0","type":"link"}