{"product_id":"neb-m0277l","title":"New England Biolabs, M0277L, AMV Reverse Transcriptase","description":"\u003cb\u003eRelated Categories\u003c\/b\u003e RT-PCR,, cDNA Synthesis \u0026amp; Reverse Transcriptases  \u003cb\u003eApplications\u003c\/b\u003e Transcription-Mediated and NASBA Amplification,, cDNA Synthesis,, Reverse Transcription (cDNA Synthesis),  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eUnit Definition\u003c\/b\u003e One unit is defined as the amount of enzyme required to incorporate 1 nmol of dTTP into an acid-insoluble form in 10 minutes at 37°C using poly(rA)-oligo(dT) as template primer.  \u003cb\u003eReaction Conditions\u003c\/b\u003e 1X AMV Reverse Transcriptase Reaction Buffer Incubate at 42°C 1X AMV Reverse Transcriptase Reaction Buffer 50 mM Tris-acetate 75 mM Potassium Acetate 8 mM Magnesium Acetate 10 mM DTT (pH 8.3 @ 25°C)  \u003cb\u003eStorage Buffer\u003c\/b\u003e 200 mM potassium phosphate 2 mM DTT 50% Glycerol 0.2% Triton® X-100 pH 7.2 @ 25°C  \u003cb\u003eHeat Inactivation\u003c\/b\u003e 85°C for 5 minutes  \u003cb\u003eUnit Assay Conditions\u003c\/b\u003e 75 mM potassium acetate, 50 mM Tris-HCl (pH 8.3) 8 mM magnesium acetate, 0.5 mM [ 3 H]-dTTP, 0.2 mM poly(rA)•oligo(dT)12–18 and 10 mM DTT. ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835477971113,"sku":"M0277L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-m0277l","provider":"Iright","version":"1.0","type":"link"}