{"product_id":"neb-m0282s","title":"New England Biolabs, M0282S, APE 1","description":"\u003cb\u003eRelated Categories\u003c\/b\u003e DNA Repair Enzymes and Structure-specific Endonucleases  \u003cb\u003eApplications\u003c\/b\u003e Polymerases for DNA Manipulation  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eUnit Definition\u003c\/b\u003e One unit is defined as the amount of enzyme required to cleave 20 pmol of a 34 mer oligonucleotide duplex containing a single AP site* in a total reaction volume of 10 μl in 1 hour at 37°C. *An AP site is created by treating 20 pmol of a 34 mer oligonucleotide duplex containing a single uracil residue with 1 unit of Uracil-DNA Glycosylase (UDG) for 2 minutes at 37°C.  \u003cb\u003eReaction Conditions\u003c\/b\u003e 1X NEBuffer™ 4 Incubate at 37°C 1X NEBuffer™ 4 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 1 mM DTT (pH 7.9 @ 25°C)  \u003cb\u003eStorage Buffer\u003c\/b\u003e 10 mM Tris-HCl 50 mM NaCl 1 mM DTT 0.05 mM EDTA 200 µg\/ml BSA 50% Glycerol pH 8 @ 25°C  \u003cb\u003eHeat Inactivation\u003c\/b\u003e 65°C for 20 minutes  \u003cb\u003eUnit Assay Conditions\u003c\/b\u003e 1X NEBuffer 4 containing 20 pmol of fluorescently labled oligonucleotide duplex in a total reaction volume of 10 μl.  \u003cb\u003eFAQ\u003c\/b\u003e Q: What is the activity of APE 1 in NEBuffers 1-4? A: APE 1 has a 100% activity in NEBuffer 4 and 50% in NEBuffer 1-3. Q: What is the molecular weight of APE 1? A: The molecular weight of Ape 1 is approximately 35 kDa. Q: Is APE 1 a tagged protein? A: No. APE 1 is not a tagged protein. Q: What substrate is used to test APE 1 activity? A: The substrates used to test our enzymes are described in the unit definition for each enzyme. APE 1 is tested using a 34-mer oligonucleotide duplex containing a single AP site. ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835493863593,"sku":"M0282S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-m0282s","provider":"Iright","version":"1.0","type":"link"}