{"product_id":"neb-m0288l","title":"New England Biolabs, M0288L, RNase HII","description":"\u003cb\u003eRelated Categories\u003c\/b\u003e RNases,, Epitranscriptome Analysis  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eUnit Definition\u003c\/b\u003e One unit is defined as the amount of enzyme required to yield a fluorescence signal consistent with the nicking of 100 picomol of synthetic double-stranded DNA substrate containing a single ribonucleotide near the quencher of a fluorophore\/quencher pair in 30 minutes at 37°C in 1X ThermoPol Buffer.  \u003cb\u003eReaction Conditions\u003c\/b\u003e 1X ThermoPol® Reaction Buffer Incubate at 37°C 1X ThermoPol® Reaction Buffer 20 mM Tris-HCl 10 mM (NH4)2SO4 10 mM KCl 2 mM MgSO4 0.1% Triton® X-100 (pH 8.8 @ 25°C)  \u003cb\u003eStorage Buffer\u003c\/b\u003e 20 mM Tris-HCl 100 mM NaCl 1 mM DTT 1 mM EDTA 50% Glycerol  \u003cb\u003eHeat Inactivation\u003c\/b\u003e No  \u003cb\u003eUnit Assay Conditions\u003c\/b\u003e 1X ThermoPol Reaction Buffer with 30 nM of a synthetic dsDNA 26-mer containing an internal ribonucleotide in a total reaction volume of 150 μl.  \u003cb\u003eFAQ\u003c\/b\u003e Q: RNase HII cannot be heat-inactivated. How can RNase HII be inactivated? A: RNase HII can be inactivated by adding to the reaction SDS to a final concentration of 0.1% Q: Which side of the ribonucleotide does RNase HII cut? A: RNase HII nicks 5’ to a ribonucleotide within the context of a DNA duplex. It leaves a 5’ phosphate and a 3’ hydroxyl end. ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835542098089,"sku":"M0288L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-m0288l","provider":"Iright","version":"1.0","type":"link"}