{"product_id":"neb-m0293s","title":"New England Biolabs, M0293S, Exonuclease I (E. coli)","description":"Note: See also Exo-CIP™ Rapid PCR Cleanup Kit (  \u003cb\u003eRelated Categories\u003c\/b\u003e Exonucleases and Non-specific Endonucleases  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eUnit Definition\u003c\/b\u003e One unit is defined as the amount of enzyme that will catalyze the release of 10 nmol of acid-soluble nucleotide in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X Exonuclease I Reaction Buffer with 0.17 mg\/ml single-stranded [ 3 H]-DNA.  \u003cb\u003eReaction Conditions\u003c\/b\u003e 1X Exonuclease I Reaction Buffer Incubate at 37°C 1X Exonuclease I Reaction Buffer 67 mM Glycine-KOH 6.7 mM MgCl2 10 mM β-ME (pH 9.5 @ 25°C)  \u003cb\u003eUsage Concentration\u003c\/b\u003e 10,000 units\/ml  \u003cb\u003eStorage Buffer\u003c\/b\u003e 10 mM Tris-HCl 100 mM NaCl 5 mM β-ME 0.5 mM EDTA 100 µg\/ml BSA 50% Glycerol pH 7.5 @ 25°C  \u003cb\u003eHeat Inactivation\u003c\/b\u003e 80°C for 20 minutes  \u003cb\u003eFAQ\u003c\/b\u003e Q: Can Exonuclease I be used to remove primers from amplification reactions? A: Exonuclease I degrades excess single stranded primer oligonucleotides from a reaction mixture containing double stranded products. Q: Will Exonuclease I degrade RNA? A: No. Q: Can Exonuclease I be used to blunt DNA? A: No. Short ends are not a substrate. Use DNA Polymerase I, Large (Klenow) Fragment (NEB# M0210) to fill in 5' overhangs (also called 3' recessed ends) and chew back 3' overhangs or use Mung Bean Nuclease (NEB# M0250) to chew back 3' or 5' overhangs. Note: 3' overhangs can not be filled in. Q: Can Exonuclease I be heat inactivated? A: Yes. Heat inactivate at 80°C for 20 minutes. Q: Will Exonuclease I work in other buffers? A: Yes. For common applications Exonuclease I will work in NEBuffers 1-4 and in the polymerase buffers. Q: Can Exonuclease I be used with a double stranded exonuclease to clean up plasmid preparations? A: Exonuclease I can be used with Lambda Exonuclease (NEB# M0262) to clean up plasmid preps. Exonuclease III (NEB# M0206) and T7 Exonuclease (NEB# M0263) will also work, but will damage nicked plasmids. Although Exonuclease I can be used, we recommend using Exonuclease V (RecBCD) (NEB #M0345) to remove chromosomal DNA after plasmid prep (see our Application Note: Using Exonuclease V (RecBCD) to Eliminate Residual Genomic DNA When Purifying Low Copy Plasmids with the Monarch® Plasmid Miniprep Kit. ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835498811561,"sku":"M0293S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-m0293s","provider":"Iright","version":"1.0","type":"link"}