{"product_id":"neb-m0297l","title":"New England Biolabs, M0297L, RNase H","description":"RNase H has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10250970.  \u003cb\u003eRelated Categories\u003c\/b\u003e RNases,, Epitranscriptome Analysis,, cDNA Synthesis \u0026amp; Reverse Transcriptases  \u003cb\u003eApplications\u003c\/b\u003e RT-PCR \u0026amp; cDNA Synthesis,, PCR  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eUnit Definition\u003c\/b\u003e One unit is defined as the amount of enzyme required to produce 1 nmol of ribonucleotides from 20 picomoles of a fluorescently labelled 50 base pair RNA-DNA hybrid in a total reaction volume of 50 μl in 20 minutes at 37°C.  \u003cb\u003eReaction Conditions\u003c\/b\u003e 1X RNase H Reaction Buffer Incubate at 37°C 1X RNase H Reaction Buffer 50 mM Tris-HCl 75 mM KCl 3 mM MgCl2 10 mM DTT (pH 8.3 @ 25°C)  \u003cb\u003eStorage Buffer\u003c\/b\u003e 50 mM KCl 10 mM Tris-HCl 0.1 mM EDTA 1 mM DTT 200 µg\/ml Recombinant Albumin 50% Glycerol pH 7.4 @ 25°C  \u003cb\u003eHeat Inactivation\u003c\/b\u003e 65°C for 20 minutes  \u003cb\u003eFAQ\u003c\/b\u003e Q: When I thaw the 10X RNase H Reaction Buffer, I see a white precipitate.  What is it and what should I do about it? A: The white precipitate is DTT in the 10X buffer, and it will resuspend when thawed and mixed. The resuspended buffer is fully functional for its intended use. Q: Is DNA Polymerase I active in RNase H buffer? A: Yes. DNA Polymerase I is active in RNase H buffer. However, for the use of both enzymes in one reaction the use of NEBuffer 2 is recommended. Q: Which side of the RNA base does RNase H cut? A: RNase H cleaves the 3'-O-P-bond of RNA. It generates 3' hydroxyl and 5' phosphate products. Q: What is the activity of RNase H at 30°C? A: The activity of RNase H at 30°C is ~90%. ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835484262569,"sku":"M0297L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-m0297l","provider":"Iright","version":"1.0","type":"link"}