{"product_id":"neb-m0298s","title":"New England Biolabs, M0298S, Cre Recombinase","description":"\u003cb\u003eRelated Categories\u003c\/b\u003e Recombinases  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eUnit Definition\u003c\/b\u003e One unit is defined as the amount of enzyme necessary to produce maximal site-specific recombination of 0.25 μg pLox2+ control DNA in 30 minutes at 37°C in a total reaction volume of 50 μl. Maximal recombination is determined by agarose gel analysis and by transformation of reactions followed by selection on ampicillin plates.  \u003cb\u003eReaction Conditions\u003c\/b\u003e 1X Cre Recombinase Reaction Buffer Incubate at 37°C 1X Cre Recombinase Reaction Buffer 33 mM NaCl 50 mM Tris-HCl 10 mM MgCl2 (pH 7.5 @ 25°C)  \u003cb\u003eUsage Concentration\u003c\/b\u003e 1,000 - 15,000 units\/ml  \u003cb\u003eStorage Buffer\u003c\/b\u003e 15 mM Tris-HCl 250 mM NaCl 0.3 mg\/ml BSA 50% Glycerol pH 8 @ 25°C  \u003cb\u003eHeat Inactivation\u003c\/b\u003e 70°C for 10 minutes  \u003cb\u003eFAQ\u003c\/b\u003e Q: What is the molecular weight of Cre Recombinase? A: 38 kD Q: Can Cre Recombinase be used to linearize a BAC containing a loxP site? A: We have not tested this here but the following paper may be helpful: Buchholz F, Bishop M. (2001). Biotechniques 31(4):906-918 \"\"LoxP-Directed cloning: use of Cre recombinase as a universal restriction enzyme.\"\" Q: What is the sequence of the loxP sites in the pLox2+ control DNA to be used with Cre Recombinase? A: 5' ATAACTTCGTATAATGTATGCTATACGAAGTTAT 3' (1) (1) Metzger, D. and Feil, R. (1999) Curr. Opin. Biotechnol. 10, 470-476. Q: Why aren't the product bands from my Cre Recombinase reaction mix sharp when run on an agarose gel? A: Incubation of the Cre Recombinase reaction mix at 70°C for 10 minutes is recommended before agarose gel analysis. This helps reduce gel artifacts. Q: Can Cre Recombinase be used to move an insert between expression vectors? A: Yes, if the insert is in a donor vector and the expression vector has been engineered to accept it. At present (10\/02) NEB does not sell a kit to make donor plasmids and none of our plasmids have been engineered to accept inserts. Check with the product manager for updates on plasmid engineering. ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835503661225,"sku":"M0298S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-m0298s","provider":"Iright","version":"1.0","type":"link"}