{"product_id":"neb-m0308s","title":"New England Biolabs, M0308S, T4 PDG (T4 Endonuclease V)","description":"\u003cb\u003eRelated Categories\u003c\/b\u003e DNA Repair Enzymes and Structure-specific Endonucleases  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eUnit Definition\u003c\/b\u003e One unit is defined as the amount of enzyme that catalyzes the conversion of 0.5 µg of UV irradiated supercoiled pUC19 DNA to \u0026gt; 95% nicked plasmid in a total reaction volume of 20 μl in 30 minutes at 37°C. Nicking is assessed by agarose gel electrophoresis. Irradiated plasmid contains an average of 3-5 pyrimidine dimers.  \u003cb\u003eReaction Conditions\u003c\/b\u003e 1X T4 PDG Reaction Buffer Supplement with 100 µg\/ml Recombinant Albumin, Molecular Biology Grade Incubate at 37°C 1X T4 PDG Reaction Buffer 100 mM NaCl 1 mM DTT 1 mM EDTA 25 mM Na2HPO4 (pH 7.2 @ 25°C)  \u003cb\u003eStorage Buffer\u003c\/b\u003e 10 mM Tris-HCl 250 mM NaCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.15% Triton® X-100 pH 7.4 @ 25°C  \u003cb\u003eHeat Inactivation\u003c\/b\u003e No  \u003cb\u003eUnit Assay Conditions\u003c\/b\u003e 1X T4 PDG Reaction Buffer containing 0.5 µg of UV irradiated supercoiled pUC19 DNA, supplemented with 100 µg\/ml Recombinant Albumin in a 20 μl reaction.  \u003cb\u003eFAQ\u003c\/b\u003e Q: What is the activity of T4 PDG in other NEBuffers, including rCutSmart? A: T4 PDG is fully active in rCutSmart and NEBuffer 4 and 75% active in NEBuffers 1-3. All buffers that do not have rAlbumin as a component will need to be supplemented with rAlbumin. Q: What is the molecular weight of T4 PDG? A: The molecular weight of T4 PDG is 16 kDa. Q: Is T4 PDG a tagged protein? A: No. T4 PDG is not a tagged protein. Q: Can the T4 PDG be used in the comet assay? A: We have not tested T4 PDG in the comet assay. Q: Does T4 PDG remove the damaged base? A: Yes. T4 PDG removes the damaged base, unlike UV Damage Endonuclease, which only nicks the DNA. Q: What type of damaged DNA does T4 PDG recognize? A: T4 PDG recognizes cis-syn-cyclobutane pyrimidine dimers caused by UV irradiation. ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835544457385,"sku":"M0308S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-m0308s","provider":"Iright","version":"1.0","type":"link"}