{"product_id":"neb-m0330s","title":"New England Biolabs, M0330S, Bsu DNA Polymerase, Large Fragment","description":"Optimal performance in a number of applications  \u003cb\u003eRelated Categories\u003c\/b\u003e DNA Manipulation  \u003cb\u003eApplications\u003c\/b\u003e Recombinase Polymerase Amplification and SIBA,, Polymerases for DNA Manipulation,, RT-PCR \u0026amp; cDNA Synthesis,  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eUnit Definition\u003c\/b\u003e One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.  \u003cb\u003eReaction Conditions\u003c\/b\u003e 1X NEBuffer™ 2 Incubate at 37°C 1X NEBuffer™ 2 50 mM NaCl 10 mM Tris-HCl 10 mM MgCl2 1 mM DTT (pH 7.9 @ 25°C)  \u003cb\u003eHeat Inactivation\u003c\/b\u003e 75°C for 20 minutes  \u003cb\u003e5' - 3' Exonuclease\u003c\/b\u003e No  \u003cb\u003e3' - 5' Exonuclease\u003c\/b\u003e No  \u003cb\u003eStrand Displacement\u003c\/b\u003e +  \u003cb\u003eUnit Assay Conditions\u003c\/b\u003e 1X NEBuffer 2, 33 µM dNTPs including [ 3 H]-dTTP and 70 µg\/ml denatured herring sperm DNA. ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835507691689,"sku":"M0330S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-m0330s","provider":"Iright","version":"1.0","type":"link"}