{"product_id":"neb-m0336s","title":"New England Biolabs, M0336S, hSMUG1","description":"\u003cb\u003eRelated Categories\u003c\/b\u003e DNA Repair Enzymes and Structure-specific Endonucleases  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eUnit Definition\u003c\/b\u003e One unit is defined as the amount of enzyme required to excizse 1 pmol of deoxyuracil from a 34 mer oligonucleotide duplex containing a single dU site in a total reaction volume of 10 μl in 1 hour at 37°C.  \u003cb\u003eReaction Conditions\u003c\/b\u003e 1X NEBuffer™ 1 Supplement with 100 µg\/ml Recombinant Albumin, Molecular Biology Grade Incubate at 37°C 1X NEBuffer™ 1 10 mM Bis-Tris-Propane-HCl 10 mM MgCl2 1 mM DTT (pH 7 @ 25°C)  \u003cb\u003eStorage Buffer\u003c\/b\u003e 10 mM Tris-HCl 250 mM NaCl 1 mM DTT 0.1 mM EDTA 200 µg\/ml Recombinant Albumin 50% Glycerol 0.15% Triton® X-100 pH 7.4 @ 25°C  \u003cb\u003eHeat Inactivation\u003c\/b\u003e 65°C for 20 minutes  \u003cb\u003eMolecular Weight\u003c\/b\u003e Theoretical: 29861 daltons  \u003cb\u003eUnit Assay Conditions\u003c\/b\u003e 1X NEBuffer 1 supplemented with 100 µg\/ml Recombinant Albumin containing 5 pmol of fluorescently labeled oligonucleotide duplex in a total reaction volume of 10 μl. After release of dU, the oligonucleotide is cleaved by treating the resulting AP site with 100 mM NaOH for 10 minutes at 80°C.  \u003cb\u003eFAQ\u003c\/b\u003e Q: What is the activity of hSMUG1 in the NEBuffers 1-4? A: hSMUG1 has full activity in NEBuffer 1, NEBuffer r1.1, NEBuffer 4 and rCutSmart. It has 25% activity in NEBuffer 3. Its use in NEBuffer 2 is not recommended. Q: What is the molecular weight of hSMUG1? A: The molecular weight of hSMUG1 is 29.86kDa. Q: What substrate is used to test hSMUG1? A: The substrates used to test enzyme activity are listed under the unit definition for each enzyme. hSMUG1 is tested using a 34-mer oligonucleotide containing a single dU site. Q: Is hSMUG1 a tagged protein? A: No. hSMUG1 is not a tagged protein. ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835522535593,"sku":"M0336S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-m0336s","provider":"Iright","version":"1.0","type":"link"}