{"product_id":"neb-m0398l","title":"New England Biolabs, M0398L, Apyrase","description":"Apyrase (recombinant,  \u003cb\u003eRelated Categories\u003c\/b\u003e Phosphatases,, RNA Modification  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eUnit Definition\u003c\/b\u003e One unit is defined as the amount of enzyme that catalyses the release of 1 μmol of inorganic phosphate from ATP (1 mM, NEB #P0756 ) in 1X Apyrase Reaction Buffer in 1 minute at 30°C in a total reaction of 50 μl.  \u003cb\u003eReaction Conditions\u003c\/b\u003e 1X Apyrase Reaction Buffer Incubate at 30°C 1X Apyrase Reaction Buffer 20 mM MES 50 mM NaCl 5 mM CaCl2 1 mM DTT 0.05% Tween® 20 (pH 6.5 @ 25°C)  \u003cb\u003eStorage Buffer\u003c\/b\u003e 20 mM MES 50 mM NaCl 0.1 mM CaCl2 1 mM DTT 0.1% Tween® 20 50% Glycerol pH 6.5 @ 25°C  \u003cb\u003eHeat Inactivation\u003c\/b\u003e 65°C for 20 minutes  \u003cb\u003eMolecular Weight\u003c\/b\u003e Apparent: 47 kDa  \u003cb\u003eSpecific Activity\u003c\/b\u003e 3,000 units\/mg  \u003cb\u003eFAQ\u003c\/b\u003e Q: What is the activity of Apyrase in other NEBuffers? A: E. coli Apyrase retains approximately 30% activity in NEBuffers r1.1, r2.1, r3.1, and rCutSmart™. Q: Can Mg2+ be substituted for Ca2+ in Apyrase reaction buffer? A: E. coli Apyrase is approximately 50% active when Mg2+ substitutes Ca2+ in Apyrase Reaction Buffer. ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835481870505,"sku":"M0398L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-m0398l","provider":"Iright","version":"1.0","type":"link"}