{"product_id":"neb-m0541l","title":"New England Biolabs, M0541L, NEBNext® High-Fidelity 2X PCR Master Mix","description":"\u003cb\u003eApplications\u003c\/b\u003e Polymerases for NGS Library Preparation,, Fast PCR,, High-Fidelity PCR,  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eHeat Inactivation\u003c\/b\u003e No  \u003cb\u003eFAQ\u003c\/b\u003e Q: What are the advantages of using NEBNext High-Fidelity 2X PCR Master Mix? A: NEBNext High-Fidelity 2X PCR Master Mix provides a unique combination of ultra-high fidelity and robust amplification of NGS libraries, regardless of GC content. Q: Is NEBNext® High-Fidelity 2X PCR Master Mix the same as the Q5® High-Fidelity Master Mix? A: No. Although both master mixes contain Q5 High-Fidelity DNA Polymerase, the master mix composition is different. NEBNext High-Fidelity 2X PCR Master Mix has been specifically optimized for amplification of NGS libraries, while Q5 High-Fidelity Master Mix has been optimized for amplification of a variety of templates. Q: Do I need to make any changes to the cycling conditions for my NGS library amplification I have previously been using with Phusion High-Fidelity PCR Master Mix? A: No. cycling conditions used successfully with Phusion High-Fidelity PCR Master Mix can also be used for NEBNext High-Fidelity 2X PCR Master Mix. Q: How many cycles of PCR should I perform with NEBNext High-Fidelity 2X PCR Master Mix? A: This depends on input amount. For use with NEBNext kits, consult the guidelines included in the product manuals. Q: Will NEBNext High-Fidelity 2X PCR Master Mix incorporate dUTPs? A: No. NEBNext High-Fidelity 2X PCR Master Mix will not incorporate dUTPs and is not compatible with bisulfite-treated DNA. For protocols employing bisulfite-treated DNA EpiMark™ Hot Start DNA Polymerase can be used. Q: Are the DNA products produced NEBNext High-Fidelity 2X PCR Master Mix blunt-ended or do they have a single base 3’ overhang? A: NEBNext High-Fidelity 2X PCR Master Mix produces blunt ends. ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835545342121,"sku":"M0541L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-m0541l","provider":"Iright","version":"1.0","type":"link"}