{"product_id":"neb-m2080s","title":"New England Biolabs, M2080S, Vaccinia Capping System","description":"GMP-grade reagent also available.  \u003cb\u003eRelated Categories\u003c\/b\u003e RNA Capping  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eUnit Definition\u003c\/b\u003e One unit of Vaccinia Capping Enzyme is defined as the amount of enzyme required to incorporate 10 pmol of (α 32 P) GTP into an 80 nt transcript in 1 hour at 37°C.  \u003cb\u003eReaction Conditions\u003c\/b\u003e 1X Capping Buffer Supplement with 0.5 mM GTP and 0.1 mM S-adenosylmethionine (SAM) Incubate at 37°C 1X Capping Buffer 50 mM Tris-HCl 5 mM KCl 1 mM MgCl2 1 mM DTT (pH 8 @ 25°C)  \u003cb\u003eStorage Buffer\u003c\/b\u003e 20 mM Tris-HCl 100 mM NaCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.1% (w\/v) Triton® X-100 pH 8 @ 25°C  \u003cb\u003eFAQ\u003c\/b\u003e Q: What is Cap-0 and Cap-1? A: Cap-0 is a N7-methyl guanosine connected to the 5′ nucleotide through a 5′ to 5′ triphosphate linkage, typically refers to as m7G cap or m7Gppp- in the literature. In the cell, the Cap-0 structure is essential for efficient translation of the mRNA that carries the cap. An additional methylation on the 2′O position of the initiating nucleotide generates Cap-1, or refers to as m7GpppNm-, where Nm denotes any nucleotide with a 2′O methylation. Cap-1 has been shown to be important in evading the cellular innate immune response in vivo. Q: Can Vaccinia Capping Enzyme (NEB #M2080) use cap analogs in capping reaction? A: No. Vaccinia Capping Enzyme uses GTP and SAM to generate a cap structure on 5′ triphosphate RNA, typically a synthetic transcript generated by in vitro transcription. On the other hand, cap analogs, such as GpppG (NEB #S1407), m7GpppG (NEB #S1404), m7GpppA (NEB #S1405S) and anti-reverse cap analog (ARCA; NEB #S1411) are used during in vitro transcription, where the cap analog is directly incorporated into the transcript as the first nucleotide at the 5′ end. ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835529810089,"sku":"M2080S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-m2080s","provider":"Iright","version":"1.0","type":"link"}