{"product_id":"neb-p0753l","title":"New England Biolabs, P0753L, Lambda Protein Phosphatase (Lambda PP)","description":"Lambda Protein Phosphatase is a Mn  \u003cb\u003eRelated Categories\u003c\/b\u003e Protein Phosphatases  \u003cb\u003eApplications\u003c\/b\u003e Protein Phosphatases and Kinases  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eUnit Definition\u003c\/b\u003e One unit is defined as the amount of enzyme that hydrolyzes 1 nmol of p -Nitrophenyl Phosphate (50 mM) (NEB #P0757) in 1 minute at 30°C in a total reaction volume of 50 μl.  \u003cb\u003eReaction Conditions\u003c\/b\u003e 1X NEBuffer Pack for Protein MetalloPhosphatases (PMP) Supplement with 1 mM MnCl2 Incubate at 30°C 1X NEBuffer Pack for Protein MetalloPhosphatases (PMP) 50 mM HEPES 100 mM NaCl 2 mM DTT 0.01% Brij 35 (pH 7.5 @ 25°C)  \u003cb\u003eStorage Buffer\u003c\/b\u003e 50 mM HEPES 100 mM NaCl 2 mM DTT 0.01% Brij 35 0.1 mM EGTA 0.1 mM MnCl2 50% Glycerol pH 7.5 @ 25°C  \u003cb\u003eHeat Inactivation\u003c\/b\u003e 65°C for 60 minutes  \u003cb\u003eMolecular Weight\u003c\/b\u003e Theoretical: 25 kDa  \u003cb\u003eSpecific Activity\u003c\/b\u003e 800,000 units\/mg  \u003cb\u003eStorage Notes\u003c\/b\u003e Avoid repeated freeze\/thaw cycles.  \u003cb\u003eShipping Notes\u003c\/b\u003e Ships on dry ice  \u003cb\u003eFAQ\u003c\/b\u003e Q: Can you recommend a cell lysis buffer that is compatible with Lambda Protein Phosphatase (NEB #P0753)? A: Lambda Protein Phosphatase (Lambda PP) is very sensitive to different reaction buffers. SDS at any concentration inhibits the enzyme. Ideally, the lysis buffer should have the closest composition to the optimal recommended buffer for Lambda PP, which is: 1X NEBuffer for PMP: 50 mM HEPES, 100 mM NaCl, 2 mM DTT, 0.01% Brij-35, pH 7.5, supplemented with 1mM MnCl2. These buffers are supplied with the enzyme. The ideal lysis buffer can also contain any mild non-ionic detergent such as 1% NP-40 or Triton X-100. If the PMP buffer is not ideal, another recommended lysis buffer is: 20 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 1 µg\/ml leupeptin, pH 7.5. ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835534004393,"sku":"P0753L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-p0753l","provider":"Iright","version":"1.0","type":"link"}