{"product_id":"neb-p7817s","title":"New England Biolabs, P7817S, RNase B","description":"\u003cb\u003eRelated Categories\u003c\/b\u003e Glycoprotein Substrates,, Proteome Analysis  \u003cb\u003eApplications\u003c\/b\u003e Protein Digestion,, Protein Labeling,, Recombinant Glycoprotein Expression,  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eStorage Buffer\u003c\/b\u003e 50 μl Milli-Q water pH 7 @ 25°C  \u003cb\u003eMolecular Weight\u003c\/b\u003e Theoretical: 17000 daltons  \u003cb\u003eFAQ\u003c\/b\u003e Q: Do detergents inhibit exoglycosidases\/endoglycosidases? A: At moderate levels (0.5-1.0% ionic and non-ionic detergents) most of the glycosidases show satisfactory activity or are unaffected. Exceptions are: PNGase F (all formulations), O-glycosidase, and β-N-Acetylglucosaminidase, which are inhibited by SDS. It is imperative to add NP-40 to a final concentration of 1% to your reaction mixture in order to counteract detergent inhibition. Endo D is also inhibited by SDS, however NP-40 does not counteract this inhibition. Finally, common stabilizing reagents such as Tween, Triton X-100, NP-40, octyl glucoside and non-detergent sulfobetaines, as well as traces of organic solvents, can prevent optimal rapid deglycosylation by Rapid PNGase F. ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835501662377,"sku":"P7817S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-p7817s","provider":"Iright","version":"1.0","type":"link"}