{"product_id":"neb-p8010l","title":"New England Biolabs, P8010L, Factor Xa Protease","description":"Factor Xa cleaves after the arginine residue in its preferred cleavage site Ile-Glu\/Asp-Gly-Arg. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate. The most common secondary site, among those that have been sequenced, is Gly-Arg.  \u003cb\u003eRelated Categories\u003c\/b\u003e Proteases  \u003cb\u003eApplications\u003c\/b\u003e Fusion Protein Cleavage,, Target Protein Insolubility ,, Protein Purification,  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eUnit Definition\u003c\/b\u003e 1 μg of Factor Xa will cleave 50 μg of MBP-fusion protein test substrate, MBP-FXa-Paramyosin-ΔSal to 95% completion in a total reaction volume of 50 μl in 6 hours or less at 25°C in 20 mM Tris-HCl (pH 8.0 @ 25°C) with 100 mM NaCl and 2 mM CaCl2.  \u003cb\u003eUsage Concentration\u003c\/b\u003e 1 mg\/ml  \u003cb\u003eRemoval\u003c\/b\u003e Factor Xa will bind specifically to benzamidine-agarose.  \u003cb\u003eStorage Buffer\u003c\/b\u003e 50 mM HEPES 500 mM NaCl 2 mM CaCl2 50% Glycerol pH 8 @ 25°C  \u003cb\u003eMolecular Weight\u003c\/b\u003e Theoretical: 43 kDa  \u003cb\u003eDeactivation\u003c\/b\u003e Dansyl-glu-gly-arg-chloromethyl ketone (CALBIOCHEM, #251700) will irreversibly inactivate Factor Xa by covalent attachment at the active site. In a reaction containing 20 μg\/ml Factor Xa, 2 μM dansyl-glu-gly-arg-chloromethyl ketone will inactivate \u0026gt;95% of the Factor Xa in 1 minute at room temperature. ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835529646249,"sku":"P8010L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-p8010l","provider":"Iright","version":"1.0","type":"link"}