{"product_id":"neb-p8070l","title":"New England Biolabs, P8070L, Enterokinase, light chain","description":"\u003cb\u003eRelated Categories\u003c\/b\u003e Proteases  \u003cb\u003eApplications\u003c\/b\u003e Fusion Protein Cleavage,, Protein Purification,, Protein Digestion  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eUnit Definition\u003c\/b\u003e 1 unit is defined as the amount of enzyme required to cleave 25 µg of a MBP-EK-paramyosin-ΔSal substrate to 95% completion in 16 hours at 25°C in a total reaction volume of 25 μl.  \u003cb\u003eRemoval\u003c\/b\u003e Enterokinase will bind specifically to trypsin inhibitor agarose.  \u003cb\u003eStorage Buffer\u003c\/b\u003e 20 mM Tris-HCl 200 mM NaCl 2 mM CaCl2 50% Glycerol pH 7.2 @ 4°C  \u003cb\u003eMolecular Weights\u003c\/b\u003e Apparent: 31 kDa Theoretical: 26.3 kDa  \u003cb\u003eUnit Assay Conditions\u003c\/b\u003e Two fold dilutions of Enterokinase, light chain are incubated with 25 µg MBP-EK-paramyosin-ΔSal substrate in 20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl 2 (pH 8.0 @ 25°C) in a 25 μl reaction. The reaction mix is incubated at 25°C for 16 hours. Separation of reaction products are visualized by SDS-PAGE. ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835477905577,"sku":"P8070L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-p8070l","provider":"Iright","version":"1.0","type":"link"}