{"product_id":"neb-r0160s","title":"New England Biolabs, R0160S, BclI","description":"BclI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10187673.  \u003cb\u003eRelated Categories\u003c\/b\u003e Restriction Endonucleases B,, Time-Saver Qualified Restriction Enzymes  \u003cb\u003eApplications\u003c\/b\u003e Restriction Enzyme Digestion  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eUnit Definition\u003c\/b\u003e One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA ( dam - ) in 1 hour at 37°C in a total reaction volume of 50 μl.  \u003cb\u003eReaction Conditions\u003c\/b\u003e 1X NEBuffer™ r3.1 Incubate at 37°C 1X NEBuffer™ r3.1 100 mM NaCl 50 mM Tris-HCl 10 mM MgCl2 100 µg\/ml Recombinant Albumin (pH 7.9 @ 25°C)  \u003cb\u003eActivity in NEBuffers\u003c\/b\u003e NEBuffer™ r1.1: 50% NEBuffer™ r2.1: 100% NEBuffer™ r3.1: 100% rCutSmart™ Buffer: 75%  \u003cb\u003eDiluent Compatibility\u003c\/b\u003e Diluent A  \u003cb\u003eStorage Buffer\u003c\/b\u003e 10 mM Tris-HCl 50 mM KCl 1 mM DTT 0.1 mM EDTA 200 µg\/ml Recombinant Albumin 50% Glycerol pH 7.4 @ 25°C  \u003cb\u003eHeat Inactivation\u003c\/b\u003e No  \u003cb\u003eMethylation Sensitivity\u003c\/b\u003e dam methylation: Blocked dcm methylation: Not Sensitive CpG Methylation: Not Sensitive  \u003cb\u003eActivity at Temperature\u003c\/b\u003e @50°C: 100%  \u003cb\u003eFAQ\u003c\/b\u003e Q: How can this enzyme be inactivated? A: To inactivate enzymes that cannot be heat killed, we recommend a phenol extraction followed by ethanol precipitation or a commercial spin column designed to purify DNA. Q: Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer? A: NEB's Time-Saver™ enzymes have the benefit of working fast (5-15 minutes), but are also designed and qualified to withstand overnight digestions without degradation of DNA. Q: Is this enzyme sensitive to dam, dcm or mammalian CpG methylation? A: Yes. This enzyme cannot cut recognition sites that are blocked by dam methylation. For up-to-date information about methylation sensitivities, please visit Dam-Dcm and CpG Methylation or REBASE. Q: Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers? A: NEB is excited to announce that we have switched BSA-containing reaction buffers (NEBuffer 1.1, 2.1, 3.1 and CutSmart® Buffer) to Recombinant Albumin-containing buffers (NEBuffer r1.1, r2.1, r3.1 and rCutSmart™ Buffer). We are also in the process of moving all restriction enzyme formulations to contain rAlbumin. We feel that moving away from animal-containing products is a step in the right direction and are able to offer this enhancement at the same price. Q: Are certain restriction enzymes more active at higher incubation temperatures than what is recommended? A: NEB restriction enzymes are 100% active at the recommended incubation temperature in the recommended buffer provided with each enzyme. With some restriction enzymes isolated from thermophilic species, you may see increased activity at higher incubation temperatures. We do not recommend increasing the temperature as additional nuclease activities may appear at non-recommended temperatures. ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835522928809,"sku":"R0160S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-r0160s","provider":"Iright","version":"1.0","type":"link"}