{"product_id":"neb-r0534l","title":"New England Biolabs, R0534L, MscI","description":"MscI has been reformulated, and now also includes Recombinant Albumin (rAlbumin), beginning with Lot #10215288.  \u003cb\u003eRelated Categories\u003c\/b\u003e Restriction Endonucleases H M  \u003cb\u003eApplications\u003c\/b\u003e Restriction Enzyme Digestion  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eUnit Definition\u003c\/b\u003e One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.  \u003cb\u003eReaction Conditions\u003c\/b\u003e 1X rCutSmart™ Buffer Incubate at 37°C 1X rCutSmart™ Buffer 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 100 µg\/ml Recombinant Albumin (pH 7.9 @ 25°C)  \u003cb\u003eActivity in NEBuffers\u003c\/b\u003e NEBuffer™ r1.1: 25% NEBuffer™ r2.1: 100% NEBuffer™ r3.1: 100% rCutSmart™ Buffer: 100%  \u003cb\u003eDiluent Compatibility\u003c\/b\u003e Diluent C  \u003cb\u003eStorage Buffer\u003c\/b\u003e 10 mM Tris-HCl 100 mM KCl 1 mM DTT 0.1 mM EDTA 0.1% Triton® X-100 50% Glycerol 200 µg\/ml Recombinant Albumin pH 7.4 @ 25°C  \u003cb\u003eHeat Inactivation\u003c\/b\u003e 80°C for 20 minutes  \u003cb\u003eMethylation Sensitivity\u003c\/b\u003e dam methylation: Not Sensitive dcm methylation: Blocked by Overlapping CpG Methylation: Not Sensitive  \u003cb\u003eFAQ\u003c\/b\u003e Q: Is MscI affected by methylation? A: MscI is blocked by overlapping dcm methylation if the site TGGCCAgg or ccTGGCCA occurs. To avoid dcm methylation, use a dcm- deficient strain for cloning such as dam-\/dcm- Competent E.coli (#C2925). For up-to-date information about methylation sensitivities, please visit Dam-Dcm and CpG Methylation or REBASE. Q: Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers? A: NEB is excited to announce that we have switched BSA-containing reaction buffers (NEBuffer 1.1, 2.1, 3.1 and CutSmart® Buffer) to Recombinant Albumin-containing buffers (NEBuffer r1.1, r2.1, r3.1 and rCutSmart™ Buffer). We are also in the process of moving all restriction enzyme formulations to contain rAlbumin. We feel that moving away from animal-containing products is a step in the right direction and are able to offer this enhancement at the same price. ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835529416873,"sku":"R0534L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-r0534l","provider":"Iright","version":"1.0","type":"link"}