{"product_id":"neb-r0635l","title":"New England Biolabs, R0635L, BseYI","description":"BseYI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10227211.  \u003cb\u003eRelated Categories\u003c\/b\u003e Restriction Endonucleases B  \u003cb\u003eApplications\u003c\/b\u003e Restriction Enzyme Digestion  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eUnit Definition\u003c\/b\u003e One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.  \u003cb\u003eReaction Conditions\u003c\/b\u003e 1X NEBuffer™ r3.1 Incubate at 37°C 1X NEBuffer™ r3.1 100 mM NaCl 50 mM Tris-HCl 10 mM MgCl2 100 µg\/ml Recombinant Albumin (pH 7.9 @ 25°C)  \u003cb\u003eActivity in NEBuffers\u003c\/b\u003e NEBuffer™ r1.1: 10% NEBuffer™ r2.1: 50% NEBuffer™ r3.1: 100% rCutSmart™ Buffer: 50%  \u003cb\u003eDiluent Compatibility\u003c\/b\u003e Diluent B  \u003cb\u003eStorage Buffer\u003c\/b\u003e 10 mM Tris-HCl 300 mM NaCl 1 mM DTT 0.1 mM EDTA 500 µg\/ml Recombinant Albumin 50% Glycerol pH 5.4 @ 25°C  \u003cb\u003eHeat Inactivation\u003c\/b\u003e 80°C for 20 minutes  \u003cb\u003eMethylation Sensitivity\u003c\/b\u003e dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked by Overlapping  \u003cb\u003eFAQ\u003c\/b\u003e Q: Is BseYI activity sensitive to dam, dcm or mammalian CpG methylation? A: Yes. BseYI cannot cut recognition sites in mammalian gDNA that contain overlapping CpG methylation. For up-to-date information about methylation sensitivities, please visit Dam-Dcm and CpG Methylation or REBASE. Q: How many base pairs should be added at the end of a PCR primer adjacent to the BseYI recognition site to guarantee that BseYI will cut properly? A: The addition of 6 base pairs is recommended. Q: Does BseYI have any isoschizomers? A: No. For the most current information about isoschizomers\/neoschizomers please refer to REBASE. Q: When I run my sample in a gel after digesting with BseYI, the DNA doesn’t seem to migrate correctly. Is there a reason for that? A: Yes. BseYI can remain bound to the DNA substrate after digestion altering the migration rate of the DNA during electrophoresis. To disrupt this binding, SDS to a final concentration of 0.5% can be added to the loading buffer prior to loading the sample in a gel. Alternatively, the DNA can be purified prior to electrophoresis. Q: Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)? A: All HF-restriction enzymes and most NEB non-HF restriction enzymes are supplied with Gel Loading Dye, Purple (6X). The non-HF restriction enzymes that come supplied with purple dye are: AatII AsiSI BsmBI-v2 EagI MboI NlaIII PvuI SmaI Acil AvrII BspHI EcoRI MboII NotI RsaI SpeI AfeI BamHI BsrGI EcoRV MluI NspI SacI StuI AflII BbsI ClaI Esp3I FseI MseI PacI SacII AgeI BglII DdeI HaeIII MspI PciI SalI XbaI AluI BsaI DpnI HindIII NcoI PmeI SapI XhoI ApaI BseYI DpnII HpaI NdeI PsiI-v2 SfaNI XmaI ApeKI BsiWI DraI KpnI NheI PstI SfiI XmnI AscI * All HF restriction enzymes are also supplied with Gel Loading Dye, Purple (6X) Q: Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers? A: NEB is excited to announce that we have switched BSA-containing reaction buffers (NEBuffer 1.1, 2.1, 3.1 and CutSmart® Buffer) to Recombinant Albumin-containing buffers (NEBuffer r1.1, r2.1, r3.1 and rCutSmart™ Buffer). We are also in the process of moving all restriction enzyme formulations to contain rAlbumin. We feel that moving away from animal-containing products is a step in the right direction and are able to offer this enhancement at the same price. ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835535544489,"sku":"R0635L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-r0635l","provider":"Iright","version":"1.0","type":"link"}