{"product_id":"neb-r0644s","title":"New England Biolabs, R0644S, Nt.BspQI","description":"\u003cb\u003eRelated Categories\u003c\/b\u003e Nicking Endonucleases,, Restriction Endonucleases: N-O  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eUnit Definition\u003c\/b\u003e One unit is defined as the amount of enzyme required to convert 1 μg of supercoiled pUC19 DNA to open circular form in 1 hour at 50°C in a total reaction volume of 50 μl.  \u003cb\u003eReaction Conditions\u003c\/b\u003e 1X NEBuffer™ r3.1 Incubate at 50°C 1X NEBuffer™ r3.1 100 mM NaCl 50 mM Tris-HCl 10 mM MgCl2 100 µg\/ml Recombinant Albumin (pH 7.9 @ 25°C)  \u003cb\u003eActivity in NEBuffers\u003c\/b\u003e NEBuffer™ r1.1: \u0026lt;10% NEBuffer™ r2.1: 25% NEBuffer™ r3.1: 100% rCutSmart™ Buffer: 10%  \u003cb\u003eDiluent Compatibility\u003c\/b\u003e Diluent B  \u003cb\u003eStorage Buffer\u003c\/b\u003e 10 mM Tris-HCl 300 mM NaCl 1 mM DTT 0.1 mM EDTA 500 µg\/ml BSA 50% Glycerol pH 7.4 @ 25°C  \u003cb\u003eHeat Inactivation\u003c\/b\u003e 80°C for 20 minutes  \u003cb\u003eMethylation Sensitivity\u003c\/b\u003e dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Not Sensitive  \u003cb\u003eActivity at Temperature\u003c\/b\u003e @37°C: 50%  \u003cb\u003eFAQ\u003c\/b\u003e Q: Are there any nicking enzymes that are used on the Irys® system from BioNano Genomics®? A: Nb.BssSI and Nt.BspQI are nicking endonucleases that cleave only one strand of DNA on a double-stranded DNA substrate. They can be used for genome mapping, assembly and SV detection on the Irys system from BioNano Genomics. For more information please visit www.BioNanogenomics.com or contact BioNano at : support@bionanogenomics.com Q: Is this enzyme sensitive to dam, dcm or mammalian CpG methylation? A: No. This enzyme is not sensitive to dam, dcm, or mammalian CpG methylation. For up-to-date information about methylation sensitivities, please visit Dam-Dcm and CpG Methylation or REBASE. Q: Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers? A: NEB is excited to announce that we have switched BSA-containing reaction buffers (NEBuffer 1.1, 2.1, 3.1 and CutSmart® Buffer) to Recombinant Albumin-containing buffers (NEBuffer r1.1, r2.1, r3.1 and rCutSmart™ Buffer). We are also in the process of moving all restriction enzyme formulations to contain rAlbumin. We feel that moving away from animal-containing products is a step in the right direction and are able to offer this enhancement at the same price. ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835485311145,"sku":"R0644S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-r0644s","provider":"Iright","version":"1.0","type":"link"}