{"product_id":"neb-r0696s","title":"New England Biolabs, R0696S, PI-SceI","description":"The large size of this product was discontinued on 12\/31\/2020. The small size will continue to be available.  \u003cb\u003eRelated Categories\u003c\/b\u003e Homing Endonucleases,, Restriction Endonucleases P R  \u003cb\u003eApplications\u003c\/b\u003e Restriction Enzyme Digestion  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eUnit Definition\u003c\/b\u003e One unit is defined as the amount of enzyme required to cleave 1 μg of pBSvdeX XmnI-linearized Control Plasmid in 3 hours at 37°C in a total reaction volume of 50 μl.  \u003cb\u003eReaction Conditions\u003c\/b\u003e 1X NEBuffer™ PI-SceI Supplement with Recombinant Albumin, Molecular Biology Grade Incubate at 37°C 1X NEBuffer™ PI-SceI 10 mM MgCl2 1 mM DTT 10 mM Tris-HCl 100 mM KCl (pH 8.6 @ 25°C)  \u003cb\u003eActivity in NEBuffers\u003c\/b\u003e NEBuffer™ r1.1: 10% NEBuffer™ r2.1: 10% NEBuffer™ r3.1: 10% rCutSmart™ Buffer: 10%  \u003cb\u003eDiluent Compatibility\u003c\/b\u003e Diluent B  \u003cb\u003eStorage Buffer\u003c\/b\u003e 10 mM Tris-HCl 1 mM DTT 0.1 mM EDTA 500 µg\/ml BSA 50% Glycerol 300 mM NaCl pH 7.4 @ 25°C  \u003cb\u003eHeat Inactivation\u003c\/b\u003e 65°C for 20 minutes  \u003cb\u003eFAQ\u003c\/b\u003e Q: Can a double digest be performed with PI-SceI and I-CeuI? A: Yes, since I-CeuI has 50% activity in PI-SceI buffer, both digestions can be done simultaneously in the unique PI-SceI buffer. Q: Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers? A: NEB is excited to announce that we have switched BSA-containing reaction buffers (NEBuffer 1.1, 2.1, 3.1 and CutSmart® Buffer) to Recombinant Albumin-containing buffers (NEBuffer r1.1, r2.1, r3.1 and rCutSmart™ Buffer). We are also in the process of moving all restriction enzyme formulations to contain rAlbumin. We feel that moving away from animal-containing products is a step in the right direction and are able to offer this enhancement at the same price. ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835540099241,"sku":"R0696S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-r0696s","provider":"Iright","version":"1.0","type":"link"}