{"product_id":"neb-t3018l","title":"New England Biolabs, T3018L, Monarch® RNase A","description":"Monarch RNase A is a component of the Monarch Genomic DNA Purification Kit (  \u003cb\u003eRelated Categories\u003c\/b\u003e RNases,, High Molecular Weight DNA Extraction,, Epitranscriptome Analysis,  \u003cb\u003eApplications\u003c\/b\u003e Nucleic Acid Purification  \u003cb\u003eSpecification\u003c\/b\u003e \u003cb\u003eStorage Buffer\u003c\/b\u003e 50 mM Tris-HCl 50% Glycerol pH 7.4  \u003cb\u003eFAQ\u003c\/b\u003e Q: Can I use a different RNase A with the Monarch® Spin gDNA Extraction Kit? A: As long as the RNase A has a similar concentration and activity specifications, it should be OK to use. However, many RNase A products do not have the same quality specifications as Monarch RNase A, which has no detectable endonuclease, exonuclease, DNase and protease activity and is free of DNA. These specifications may be relevant for your downstream applications. Q: Can I omit the RNase A digestion step from the protocol? A: Yes, the Monarch gDNA purification procedure has been designed to minimize the RNA amount that is co-purified. Typical percentages of RNA that are found in gDNA Monarch eluates when the RNase A digestion step is omitted are 1% (Blood Protocol), 10% (Cell Protocol), and 1-5% (Tissue Protocol). These low amounts typically do not affect most downstream applications. If the RNase A digestion step is included in each protocol, final RNA amounts that are co-purified with the gDNA are 0-1%, amounts that are not detectable by most methods. Q: Can I use Monarch® RNase A for applications\/workflows other than gDNA purification? A: Although NEB has not specifically validated the use of Monarch RNase A in applications outside of gDNA purification, the enzyme should work well for other applications that utilize RNase A. Q: The enzymes in the kit were not stored at -20°C right away. Will they still work? A: Yes, unopened Monarch Proteinase K and RNAse A enzymes are stable at ambient temperatures short-term. After opening, Proteinase K and Monarch RNAse A should be stored at -20°C. Q: Is Monarch RNase A included with Monarch Buffer B1? A: No, Monarch Buffer B1 is not supplied with Monarch RNase A. Monarch RNase A (NEB #T3018) is available for purchase separately. Monarch Buffer B1 requires addition of Monarch RNase A for plasmid miniprep applications. Refer to appropriate buffer preparation instructions for your specific workflow before use. ","brand":"New England Biolabs","offers":[{"title":"Default Title","offer_id":46835534037161,"sku":"T3018L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/neb-t3018l","provider":"Iright","version":"1.0","type":"link"}