{"product_id":"promega-cs1810e24","title":"Promega, CS1810E24, NanoBRET® TE Intracellular E3 Ligase Assays","description":"\u003cp\u003eMeasure E3 Ligase Target Engagement and Compound Intracellular Availability\u003cbr\u003e\nQuantitate intracellular affinity of compounds for CRBN or VHL E3 ubiquitin ligases in live cells\u003cbr\u003e\nExecute a simple workflow to calculate compound intracellular availability, a measure of permeability, to aid in compound prioritization\u003cbr\u003e\nScalable assays using addition-only protocols\u003cbr\u003e\nSpecifications：\u003cbr\u003e\nNanoBRET® Nano-Glo® Substrate\tN157A\t1 × 50μl\u003cbr\u003e\nTracer Dilution Buffer\tN219A\t1 × 5ml\u003cbr\u003e\nExtracellular NanoLuc® Inhibitor\tN235A\t1 × 17μl\u003cbr\u003e\nNanoLuc®-CRBN Fusion Vector\tN274A\t1 × 20μg\u003cbr\u003e\nDDB1 Expression Vector\tN276A\t1 × 20μg\u003cbr\u003e\nNanoBRET® TE Tracer CRBN\tN290A\t1 × 30μl\u003cbr\u003e\nNanoBRET® Nano-Glo® Substrate\tN157C\t1 × 330μl\u003cbr\u003e\nTracer Dilution Buffer\tN219A\t1 × 5ml\u003cbr\u003e\nExtracellular NanoLuc® Inhibitor\tN235B\t1 × 110μl\u003cbr\u003e\nNanoLuc®-CRBN Fusion Vector\tN274A\t1 × 20μg\u003cbr\u003e\nDDB1 Expression Vector\tN276A\t3 × 20μg\u003cbr\u003e\nNanoBRET® TE Tracer CRBN\tN290B\t1 × 300μl\u003cbr\u003e\nNanoBRET® Nano-Glo® Substrate\tN157D\t1 × 3.3ml\u003cbr\u003e\nTracer Dilution Buffer\tN219B\t1 × 50ml\u003cbr\u003e\nExtracellular NanoLuc® Inhibitor\tN235C\t1 × 1.1ml\u003cbr\u003e\nNanoBRET® TE Tracer CRBN\tN290B\t10 × 300μl\u003cbr\u003e\nTransfection Carrier DNA\tE488A\t1 × 20μg\u003cbr\u003e\nNanoBRET® Nano-Glo® Substrate\tN157A\t1 × 50μl\u003cbr\u003e\nTracer Dilution Buffer\tN219A\t1 × 5ml\u003cbr\u003e\nExtracellular NanoLuc® Inhibitor\tN235A\t1 × 17μl\u003cbr\u003e\nVHL-NanoLuc® Fusion Vector\tN275A\t1 × 20μg\u003cbr\u003e\nNanoBRET® TE Tracer VHL\tN292A\t1 × 30μl\u003cbr\u003e\nTransfection Carrier DNA\tE488B\t1 × 100μg\u003cbr\u003e\nNanoBRET® Nano-Glo® Substrate\tN157C\t1 × 330μl\u003cbr\u003e\nTracer Dilution Buffer\tN219A\t1 × 5ml\u003cbr\u003e\nExtracellular NanoLuc® Inhibitor\tN235B\t1 × 110μl\u003cbr\u003e\nVHL-NanoLuc® Fusion Vector\tN275A\t1 × 20μg\u003cbr\u003e\nNanoBRET® TE Tracer VHL\tN292B\t1 × 300μl\u003cbr\u003e\nNanoBRET® Nano-Glo® Substrate\tN157D\t1 × 3.3ml\u003cbr\u003e\nTracer Dilution Buffer\tN219B\t1 × 50ml\u003cbr\u003e\nExtracellular NanoLuc® Inhibitor\tN235C\t1 × 1.1ml\u003cbr\u003e\nNanoBRET® TE Tracer VHL\tN292B\t10 × 300μl\u003cbr\u003e\nProtein degradation via small molecules such as PROTACs or molecular glues is governed by multiple steps. The\u003cbr\u003e\ninitial steps involve entry of the small molecule degrader compound into the cell, followed by binding to the E3\u003cbr\u003e\nubiquitin ligase and protein target. The NanoBRET® TE Intracellular E3 Ligase Assays quantitate both intracellular\u003cbr\u003e\ntarget engagement and intracellular degrader compound availability.\u003cbr\u003e\nThese assays are based on the NanoBRET® technology, an energy transfer technique designed to measure molecular\u003cbr\u003e\nproximity in living cells. S\u003cbr\u003e\npecifically, the NanoBRET®\u003cbr\u003e\nTarget Engagement (TE) Intracellular E3 Ligase Assays allow live cell measurement of degrader compound affinity for an E3 ubiquitin ligase protein, Cereblon (CRBN) or von Hippel-Lindau disease tumor suppressor (VHL).\u003cbr\u003e\nI\u003cbr\u003e\nn addition, the assays can be used to quantify intracellular availability of a compound.\u003cbr\u003e\nAs heterobifunctional degraders like PROTACs often have high molecular weight, they may suffer from poor cell\u003cbr\u003e\npermeability or intracellular availability. By performing a simple workflow involving two unique assay modes (live-cell and permeabilized-cell),  the NanoBRET®\u003cbr\u003e\nTE E3 Ligase Assays can be used to calculate the intracellular availability of a degrader compound.\u003c\/p\u003e","brand":"Promega","offers":[{"title":"Default Title","offer_id":46835696533673,"sku":"CS1810E24","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/promega-cs1810e24","provider":"Iright","version":"1.0","type":"link"}