{"product_id":"promega-g9062","title":"Promega, G9062, NAD(P)H-Glo™ Detection System","description":"\u003cp\u003eMonitor the Concentration of Reduced Forms of NADH and NADPH\u003cbr\u003e\nHighly sensitive and rapid bioluminescent assay\u003cbr\u003e\nEasily adapted for high-throughput format\u003cbr\u003e\nSimple add-mix-measure protocol\u003cbr\u003e\nSpecifications：\u003cbr\u003e\nReductase\tG884A\t1 × 55μl\u003cbr\u003e\nReductase Substrate\tG885A\t1 × 55μl\u003cbr\u003e\nReductase\tG884B\t1 × 275μl\u003cbr\u003e\nReductase Substrate\tG885B\t1 × 275μl\u003cbr\u003e\nThe NAD(P)H-Glo™ Detection System is a bioluminescent, homogeneous, single-reagent-addition assay that quantitatively monitors the concentration of the reduced forms of NADH and NADPH levels in enzymatic reactions. The oxidized forms, NAD+ and NADP+, are not detected and do not interfere with quantitation.\u003cbr\u003e\nThe assay is rapid, requiring only a 40- to 60-minute incubation, has a broad linear range (25nM-50µM) and high signal-to background ratio (~400). Low nanomolar sensitivity, 25nM, allows direct in-well detection. The assay is well suited to measuring NAD(P)H production or consumption in high-throughput formats.\u003cbr\u003e\nIn the presence of NAD(P)H, a reductase reduces the proluciferin reductase substrate to form luciferin. Luciferin is quantified using Ultra-Glo™ Recombinant Luciferase, which is a component of the Luciferin Detection Reagent, and the light signal produced is proportional to the amount of NAD(P)H in the sample.\u003c\/p\u003e","brand":"Promega","offers":[{"title":"Default Title","offer_id":46835664552105,"sku":"G9062","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/promega-g9062","provider":"Iright","version":"1.0","type":"link"}