{"product_id":"promega-ga1230","title":"Promega, GA1230, T Cell Activation Bioassay (TCRαβ-KO)","description":"\u003cp\u003eTools to Screen and Characterize Transgenic TCR\u003cbr\u003e\nIdentify TCRs specific for a peptide\/HLA of interest\u003cbr\u003e\nTest specificity of candidate TCR\u003cbr\u003e\nSpecifications：\u003cbr\u003e\nTCRαβ-KO (CD4+) Cells (CPM)\tGA117A\t2 × 1ml\u003cbr\u003e\nTCRαβ-KO (CD8+) Cells (CPM)\tGA116A\t2 × 1ml\u003cbr\u003e\nTCRαβ-KO (CD4+, CD8+) (CPM)\tGA118A\t2 × 1ml\u003cbr\u003e\nTCRαβ-KO (CD4+) Cells (CPM)\tGA117A\t50 × 1ml\u003cbr\u003e\nTCRαβ-KO (CD8+) Cells (CPM)\tGA116A\t50 × 1ml\u003cbr\u003e\nTCRαβ-KO (CD4+, CD8+) (CPM)\tGA118A\t50 × 1ml\u003cbr\u003e\nAdoptive T cell immunotherapy is an important cancer treatment strategy in which genetically modified T cells are made tumor-specific and activated via introduction of a transgenic antigen receptor, which improves their ability to recognize and destroy tumors. T cell receptor-engineered T cell (TCR-T) and chimeric antigen receptor T cell (CAR-T) therapy are two types of adoptive T cell immunotherapy.\u003cbr\u003e\nMeasurement of TCR activation using traditional methods relies on laborious protocols and highly variable end points. The TCRαβ-KO Bioassay overcomes the limitations of these existing methods and enables functional testing of transgenic TCRs for antigen ranking, specificity and safety testing for applications such as quality control, TCR-T and CAR-T therapies.\u003cbr\u003e\nThe T Cell Activation Bioassay (TCRαβ-KO) is a bioluminescent cell-based assay for measuring the potency of transgenic TCR constructs to activate T cells without the constraints of endogenous TCR expression. The assay consists of a genetically engineered Jurkat T cell line with endogenous TCR α and β chains knocked out using CRISPR\/Cas9. These cells express a luciferase reporter driven by a TCR pathway-dependent promoter.\u003cbr\u003e\nTCRαβ-KO Bioassay is available in CPM and Cell Bank formats:\u003cbr\u003e\nThe TCR Transfection Positive Control Kit is available separately. If interested, please note as part of your inquiry.\u003c\/p\u003e","brand":"Promega","offers":[{"title":"Default Title","offer_id":46835631390889,"sku":"GA1230","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/promega-ga1230","provider":"Iright","version":"1.0","type":"link"}