{"product_id":"promega-ga1345","title":"Promega, GA1345, FcγRI ADCP Bioassay","description":"\u003cp\u003eMeasure Potency and Stability of Biologics that Bind and Activate ADCP through FcγRI\u003cbr\u003e\nPrequalified according to ICH guidelines\u003cbr\u003e\nNo primary cell culture required\u003cbr\u003e\nSupplied in convenient thaw-and-use format; also available in cell propagation model (CPM) format\u003cbr\u003e\nSpecifications：\u003cbr\u003e\nRPMI 1640 Medium\tG708A\t1 × 36ml\u003cbr\u003e\nLow IgG Serum\tG711A\t1 × 4ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Buffer\tG719A\t1 × 10ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Substrate\tG720A\t1 × 1 vial\u003cbr\u003e\nFcγRI ADCP Effector Cells\tGA133A\t1 × 0.7ml\u003cbr\u003e\nRPMI 1640 Medium\tG708A\t5 × 36ml\u003cbr\u003e\nLow IgG Serum\tG711A\t5 × 4ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Buffer\tG719A\t5 × 10ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Substrate\tG720A\t5 × 1 vial\u003cbr\u003e\nFcγRI ADCP Effector Cells\tGA133A\t5 × 0.7ml\u003cbr\u003e\nFcγRI ADCP Effector Cells (CPM)\tGA133B\t2 × 1ml\u003cbr\u003e\nFcγRI ADCP Effector Cells (CPM)\tGA133B\t50 × 1ml\u003cbr\u003e\nFc receptor-mediated antibody-dependent cell-mediated phagocytosis (ADCP) is an important mechanism of action (MOA) of therapeutic antibodies designed to recognize and mediate the elimination of virus-infected or diseased (e.g., tumor) cells. Unlike antibody-dependent cell-mediated cytotoxicity (ADCC), which is mediated primarily through FcγRIIIa expressed on NK cells, ADCP can be mediated by monocytes, macrophages, neutrophils and dendritic cells via FcγRIIa, FcγRIIIa and FcγRI. All three receptors can participate in antibody recognition, receptor clustering and signaling events that result in ADCP.\u003cbr\u003e\nCurrent methods used to measure ADCP rely on the isolation of primary human monocytes, ex vivo differentiation into macrophages and measurement of target cell engulfment. These assays are laborious and highly variable due to complex assay protocols and unqualified assay reagents. As a result, these current methods are difficult to establish in quality-controlled, drug development settings.\u003cbr\u003e\nThe FcγRI ADCP Bioassay is a bioluminescent cell-based assay for measuring the potency and stability of antibodies and other biologics with Fc domains that bind and activate FcγRI. The assay consists of a genetically engineered Jurkat T cell line that expresses the high-affinity human FcγRI and a luciferase reporter. The bioassay workflow is simple, robust, consistent and overcomes many of the limitations of traditional methods.\u003c\/p\u003e","brand":"Promega","offers":[{"title":"Default Title","offer_id":46835623985321,"sku":"GA1345","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/promega-ga1345","provider":"Iright","version":"1.0","type":"link"}