{"product_id":"promega-j1195","title":"Promega, J1195, PD-1\/PD-L1 Blockade Bioassay","description":"\u003cp\u003eImmune Checkpoint Bioassays for Characterizing Immune-Modulating Antibodies\u003cbr\u003e\nPrequalified according to ICH guidelines\u003cbr\u003e\n96- and 384-well plate formats\u003cbr\u003e\nNo primary cell culture required\u003cbr\u003e\nSupplied in convenient thaw-and-use format; also available in cell propagation model (CPM) format\u003cbr\u003e\nSpecifications：\u003cbr\u003e\nPD-1 Effector Cells\tJ115A\t1 × 0.5ml\u003cbr\u003e\nPD-L1 aAPC\/CHO-K1 Cells\tJ109A\t1 × 0.5ml\u003cbr\u003e\nFetal Bovine Serum\tJ121A\t1 × 4ml\u003cbr\u003e\nHam's F-12 Medium\tJ123A\t1 × 25ml\u003cbr\u003e\nRPMI 1640 Medium\tG708A\t1 × 36ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Buffer\tG719A\t1 × 10ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Substrate\tG720A\t1 × 1 vial\u003cbr\u003e\nPD-1 Effector Cells\tJ115A\t5 × 0.5ml\u003cbr\u003e\nPD-L1 aAPC\/CHO-K1 Cells\tJ109A\t5 × 0.5ml\u003cbr\u003e\nFetal Bovine Serum\tJ121A\t5 × 4ml\u003cbr\u003e\nHam's F-12 Medium\tJ123A\t5 × 25ml\u003cbr\u003e\nRPMI 1640 Medium\tG708A\t5 × 36ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Buffer\tG719A\t5 × 10ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Substrate\tG720A\t5 × 1 vial\u003cbr\u003e\nPD-1 Effector Cells (CPM)\tJ112A\t2 × 1ml\u003cbr\u003e\nPD-L1 aAPC\/CHO-K1 Cells (CPM)\tJ108A\t2 × 1ml\u003cbr\u003e\naAPC\/CHO-K1 Cells\tJ119A\t1 × 0.5ml\u003cbr\u003e\naAPC\/CHO-K1 Cells\tJ119A\t5 × 0.5ml\u003cbr\u003e\nControl Ab, Anti-PD-1\tJ120A\t1 × 100μg\u003cbr\u003e\nPD-1 is an immune inhibitory receptor expressed on activated T cells and B cells and plays a critical role in regulating immune responses to tumor antigens and autoantigens. Engagement of PD-1 by either of its ligands, PD-L1 or PD-L2, on an adjacent cell inhibits TCR signaling and TCR-mediated proliferation, transcriptional activation and cytokine production. Therapeutic antibodies and Fc fusion proteins designed to block the PD-1\/PD-L1 interaction show promising results in clinical trials for the treatment of a variety of cancers.\u003cbr\u003e\nThe PD-1\/PD-L1 Blockade Bioassay is a biologically relevant MOA-based assay that can be used to measure the potency and stability of antibodies and other biologics designed to block the PD-1\/PD-L1 interaction.\u003cbr\u003e\nControl Ab, Anti-PD-1, is a mouse anti-human PD-1 antibody that shows blocking activity for PD-1 with its ligands PD-L1 and PD-L2. It is intended for use as a control with the PD-1\/PD-L1 Blockade Bioassay.\u003cbr\u003e\naAPC\/CHO-K1 Cells are CHO-K1 cells with an engineered cell surface protein designed to activate cognate TCRs in an antigen-independent manner. The PD-L1 Negative aAPC\/CHO-K1 Cells can serve as a negative control in the PD-1\/PD-L1 Blockade Bioassay.\u003c\/p\u003e","brand":"Promega","offers":[{"title":"Default Title","offer_id":46835630964905,"sku":"J1195","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/promega-j1195","provider":"Iright","version":"1.0","type":"link"}