{"product_id":"promega-j2952","title":"Promega, J2952, IL-2 Bioassay","description":"\u003cp\u003eA Simpler, More Consistent IL-2 Bioassay\u003cbr\u003e\nPrequalified according to ICH guidelines\u003cbr\u003e\nMechanism-of-action-based assay\u003cbr\u003e\nNo primary cell culture required\u003cbr\u003e\nSupplied in convenient thaw-and-use format; also available in cell propagation model (CPM) format\u003cbr\u003e\nSpecifications：\u003cbr\u003e\nIL-2 Bioassay Cells\tJ297A\t1 × 0.80ml\u003cbr\u003e\nFetal Bovine Serum\tJ121A\t1 × 4ml\u003cbr\u003e\nRPMI 1640 Medium\tG708A\t1 × 36ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Buffer\tG719A\t1 × 10ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Substrate\tG720A\t1 × 1 vial\u003cbr\u003e\nIL-2 Bioassay Cells\tJ297A\t5 × 0.80ml\u003cbr\u003e\nFetal Bovine Serum\tJ121A\t5 × 4ml\u003cbr\u003e\nRPMI 1640 Medium\tG708A\t5 × 36ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Buffer\tG719A\t5 × 10ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Substrate\tG720A\t5 × 1 vial\u003cbr\u003e\nIL-2 Bioassay Cells (CPM)\tJ295A\t2 × 0.75ml\u003cbr\u003e\nInterleukin-2 (IL-2), originally described as “T-cell growth factor” in 1976, is a small 15.5kDa monomer secreted by a variety of cell types including CD4+ and CD8+ T cells, natural killer (NK) cells and activated dendritic cells. IL-2 has pleiotropic effects on the immune system. It plays a critical role in the generation, maintenance and expansion of CD4+ regulatory T cells, promotes the cytotoxic activity of NK and CD8+ cells and governs homeostasis through the elimination of harmful autoreactive T cells via activation-induced cell death.\u003cbr\u003e\nThe IL-2 Bioassay is a bioluminescent cell-based assay designed to measure IL-2 stimulation or inhibition. The assay overcomes many of the limitations of the current endothelial cell proliferation assays and can be used for the discovery and development of novel biologic therapies aimed at either inducing or inhibiting the IL-2 response. The IL-2 Bioassay Cells have been engineered to express\u003cbr\u003e\nluc2\u003cbr\u003e\nin response to IL-2 signaling. When IL-2 binds to IL-2 Bioassay Cells, the receptor transduces intracellular signals that result in luminescence. The bioluminescent signal is detected and quantified using Bio-Glo™ Luciferase Assay System (Cat.# G7940) and a standard luminometer such as the GloMax® Discover System (Cat.# GM3000).\u003c\/p\u003e","brand":"Promega","offers":[{"title":"Default Title","offer_id":46835622117545,"sku":"J2952","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/promega-j2952","provider":"Iright","version":"1.0","type":"link"}