{"product_id":"promega-j2962","title":"Promega, J2962, IL-15 Bioassay","description":"\u003cp\u003eA Simpler, More Consistent IL-15 Bioassay\u003cbr\u003e\nPrequalified according to ICH guidelines\u003cbr\u003e\nMechanism-of-action-based assay\u003cbr\u003e\nNo primary cell culture required\u003cbr\u003e\nSupplied in convenient thaw-and-use format; also available in cell propagation model (CPM) format\u003cbr\u003e\nSpecifications：\u003cbr\u003e\nIL-15 Bioassay Cells\tJ298A\t1 × 0.80ml\u003cbr\u003e\nFetal Bovine Serum\tJ121A\t1 × 4ml\u003cbr\u003e\nRPMI 1640 Medium\tG708A\t1 × 36ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Buffer\tG719A\t1 × 10ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Substrate\tG720A\t1 × 1 vial\u003cbr\u003e\nIL-15 Bioassay Cells\tJ298A\t5 × 0.80ml\u003cbr\u003e\nFetal Bovine Serum\tJ121A\t5 × 4ml\u003cbr\u003e\nRPMI 1640 Medium\tG708A\t5 × 36ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Buffer\tG719A\t5 × 10ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Substrate\tG720A\t5 × 1 vial\u003cbr\u003e\nIL-15 Bioassay Cells (CPM)\tJ296A\t2 × 0.75ml\u003cbr\u003e\nInterleukin-15 (IL-15) is secreted by a variety of cell types including monocytes, macrophages, dendritic and epithelial cells. IL-15 promotes activation and expansion of natural killer (NK), natural killer T (NKT) and CD8+ memory T cells. Unlike IL-2, IL-15 doesn’t impact CD4+ regulatory T cells (Treg) or induce activation-induced cell death (AICD).\u003cbr\u003e\nMultiple pathways can be activated by IL-15 signaling. In lymphocytes, JAK\/STAT signaling begins with JAK1 and JAK3 tyrosine kinase recruitment and activation at the receptor cytoplasmic domains. These kinases recruit and activate STAT3 and 5 with phosphorylated dimer\/tetramer translocation to the nucleus for transcriptional activation of a variety of proteins. These pathways impact cell proliferation, anti-apoptotic survival and cytotoxic effector functions.\u003cbr\u003e\nThe IL-15 Bioassay is a bioluminescent cell-based assay designed to measure IL-15 stimulation or inhibition. The IL-15 Bioassay Cells have been engineered to express\u003cbr\u003e\nluc2\u003cbr\u003e\nin response to IL-15 signaling. When IL-15 binds to IL-15 Bioassay Cells, the receptor transduces intracellular signals resulting in luminescence. The bioluminescent signal is detected and quantified using Bio-Glo™ Luciferase Assay System (Cat.# G7940) and a standard luminometer such as the GloMax® Discover System (Cat.# GM3000).\u003c\/p\u003e","brand":"Promega","offers":[{"title":"Default Title","offer_id":46835622019241,"sku":"J2962","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/promega-j2962","provider":"Iright","version":"1.0","type":"link"}