{"product_id":"promega-j3002","title":"Promega, J3002, IL-23 Bioassay","description":"\u003cp\u003eA Simpler, More Consistent IL-23 Bioassay\u003cbr\u003e\nPrequalified according to ICH guidelines\u003cbr\u003e\n96- and 384-well plate formats\u003cbr\u003e\nNo primary cell culture required\u003cbr\u003e\nSupplied in convenient thaw-and-use format; also available in cell propagation model (CPM) format\u003cbr\u003e\nSpecifications：\u003cbr\u003e\nIL-23 Bioassay Cells (CPM)\tJ300A\t2 × 0.65ml\u003cbr\u003e\nIL-23 Bioassay Cells\tJ303A\t1 × 0.65ml\u003cbr\u003e\nFetal Bovine Serum\tJ121A\t1 × 4ml\u003cbr\u003e\nRPMI 1640 Medium\tG708A\t1 × 36ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Buffer\tG719A\t1 × 10ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Substrate\tG720A\t1 × 1 vial\u003cbr\u003e\nIL-23 Bioassay Cells\tJ303A\t5 × 0.65ml\u003cbr\u003e\nFetal Bovine Serum\tJ121A\t5 × 4ml\u003cbr\u003e\nRPMI 1640 Medium\tG708A\t5 × 36ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Buffer\tG719A\t5 × 10ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Substrate\tG720A\t5 × 1 vial\u003cbr\u003e\nInterleukin 23 (IL-23) is a member of the IL-12 cytokine family, which consists of IL-12, IL-23, IL-27, IL-35 and IL-39. IL-23 binds and signals through a heterodimeric receptor complex. The IL-23 receptor is found on natural killer cells, macrophages, memory T cells (Th17) and keratinocytes. In response to microbial pathogens and wound healing signals, IL-23 is secreted by activated dendritic cells and macrophages with subsequent neutrophil recruitment. Upon IL-23 binding to Th17 cells, signaling begins with tyrosine kinase 2 (TYK2) recruitment to IL-12Rβ1 and Janus kinase 2 (JAK2) recruitment to IL-23R. These kinases phosphorylate and activate signal transducer and activator of transcription 3 (STAT3), and to a lesser extent STAT4, STAT1 and STAT5.\u003cbr\u003e\nThe IL-23 Bioassay is a bioluminescent cell-based assay designed to measure IL-23 stimulation or inhibition. IL-23 Bioassay Cells have been engineered to express luc2P in response to IL-23 signaling. When IL-23 binds to IL-23 Bioassay Cells, the receptor transduces intracellular signals resulting in luminescence. The bioluminescent signal is detected and quantified using Bio-Glo™ Reagent.\u003c\/p\u003e","brand":"Promega","offers":[{"title":"Default Title","offer_id":46835621822633,"sku":"J3002","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/promega-j3002","provider":"Iright","version":"1.0","type":"link"}