{"product_id":"promega-j3042","title":"Promega, J3042, IL-12 Bioassay","description":"\u003cp\u003eA Simpler, More Consistent IL-12 Bioassay\u003cbr\u003e\nPrequalified according to ICH guidelines\u003cbr\u003e\n96- and 384-well plate formats\u003cbr\u003e\nNo primary cell culture required\u003cbr\u003e\nSupplied in convenient thaw-and-use format; also available in cell propagation model (CPM) format\u003cbr\u003e\nSpecifications：\u003cbr\u003e\nIL-12 Bioassay Cells (CPM)\tJ304A\t2 × 0.65ml\u003cbr\u003e\nIL-12 Bioassay Cells\tJ305A\t1 × 0.65ml\u003cbr\u003e\nFetal Bovine Serum\tJ121A\t1 × 4ml\u003cbr\u003e\nRPMI 1640 Medium\tG708A\t1 × 36ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Buffer\tG719A\t1 × 10ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Substrate\tG720A\t1 × 1 vial\u003cbr\u003e\nIL-12 Bioassay Cells\tJ305A\t5 × 0.65ml\u003cbr\u003e\nFetal Bovine Serum\tJ121A\t5 × 4ml\u003cbr\u003e\nRPMI 1640 Medium\tG708A\t5 × 36ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Buffer\tG719A\t5 × 10ml\u003cbr\u003e\nBio-Glo™ Luciferase Assay Substrate\tG720A\t5 × 1 vial\u003cbr\u003e\nInterleukin-12 (IL-12) is a member of the IL-12 cytokine family, which includes IL-12, IL-23, IL-27, IL-35 and IL-39. Both IL-12 and IL-23 are composite cytokines, sharing a common IL-12 p40 subunit and IL-12Rβ1 receptor. Cytokine specificity is derived from the unique IL-12 p35 subunit binding to IL-12Rβ2 and the IL-23p19 subunit binding to IL-23R. Both p35 and p40 genes need to be expressed within the same cell to produce the active heterodimer and subsequent IL-12 signaling. IL-12 is secreted following stimulation of phagocytes and dendritic cells by bacteria and other microorganisms.\u003cbr\u003e\nThe IL-12 Bioassay is a bioluminescent cell-based assay designed to measure IL-12 stimulation or inhibition. The IL-12 Bioassay Cells have been engineered to express luc2P in response to IL-12 signaling. When IL-12 binds to IL-12 Bioassay Cells, the receptor transduces intracellular signals resulting in luminescence. The bioluminescent signal is detected and quantified using Bio-Glo™ Reagent.\u003c\/p\u003e","brand":"Promega","offers":[{"title":"Default Title","offer_id":46835621691561,"sku":"J3042","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/promega-j3042","provider":"Iright","version":"1.0","type":"link"}