{"product_id":"promega-ja5105","title":"Promega, JA5105, IL-2Rβγ Bioassay","description":"\u003cp\u003eAssess the Mechanism of Action of Cytokine Biologics Designed to Target the Intermediate Affinity IL-2Rβγ Receptor\u003cbr\u003e\nLacks CD25 expression\u003cbr\u003e\nPrequalified according to ICH guidelines\u003cbr\u003e\n96- and 384-well plate formats\u003cbr\u003e\nNo primary cell culture required\u003cbr\u003e\nSupplied in convenient thaw-and-use format; also available in cell propagation model (CPM) format\u003cbr\u003e\nSpecifications：\u003cbr\u003e\nIL-2Rβγ Bioassay Cells\tJ340A\t1 × 0.6ml\u003cbr\u003e\nFetal Bovine Serum\tJ121A\t1 × 4ml\u003cbr\u003e\nRPMI 1640 Medium\tG708A\t1 × 36ml\u003cbr\u003e\nBio-Glo-NL™ Luciferase Assay Buffer\tJ308A\t1 × 10ml\u003cbr\u003e\nBio-Glo-NL™ Luciferase Assay Substrate\tJ309A\t1 × 200μl\u003cbr\u003e\nIL-2Rβγ Bioassay Cells\tJ340A\t5 × 0.6ml\u003cbr\u003e\nFetal Bovine Serum\tJ121A\t5 × 4ml\u003cbr\u003e\nRPMI 1640 Medium\tG708A\t5 × 36ml\u003cbr\u003e\nBio-Glo-NL™ Luciferase Assay Buffer\tJ308A\t5 × 10ml\u003cbr\u003e\nBio-Glo-NL™ Luciferase Assay Substrate\tJ309A\t5 × 200μl\u003cbr\u003e\nIL-2Rβγ Bioassay Cells (CPM)\tJ339A\t2 × 1ml\u003cbr\u003e\nIL-2Rβγ Bioassay Cells (CPM)\tJ339A\t50 × 1ml\u003cbr\u003e\nThe IL-2 receptor (IL-2R) is a type 1 cytokine receptor consisting of three subunits: IL-2Rα (CD25), IL-2\/IL-15Rβ (CD122) and IL-2Rγ (CD132). The high-affinity trimeric receptor form consists of all three subunits and is found on regulatory T cells (Treg), activated CD4 and CD8 effector T cells and some natural killer (NK) cells, as well as various endothelial cell types. A medium-affinity heterodimeric receptor form, containing only IL-2Rβ and IL-2Rβγ, is found on CD8+ effector memory T cells and most NK cells. IL-2Rγ is also known as the γ common chain (γc) and is found in receptors for IL-4, IL-7, IL-9, IL-15 and IL-21.\u003cbr\u003e\nHigh-dose recombinant IL-2 therapy can promote the expansion and development of immunosuppressive Treg cells, which are generally regarded as undesirable in the tumor microenvironment. IL-15 is a functionally related cytokine; however, unlike IL-2, it has no effect on Treg cells. To avoid the pitfalls of high-dose IL-2, other IL-2 and IL-15 molecules are in development that target the medium-affinity receptor and cell types that express it, which may produce a safer and more specific therapeutic response.\u003cbr\u003e\nThe IL-2Rβγ Bioassay is a bioluminescent cell-based assay designed to measure human IL-2Rβγ receptor stimulation or inhibition. The assay overcomes many of the limitations of current methods that cannot differentiate between signaling through high- and medium-affinity IL-2 receptors. These cells can be used for the development of biologic therapies intended to preferentially target the intermediate affinity heterodimeric receptor.\u003c\/p\u003e","brand":"Promega","offers":[{"title":"Default Title","offer_id":46835610878121,"sku":"JA5105","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/promega-ja5105","provider":"Iright","version":"1.0","type":"link"}