{"product_id":"promega-ja9011","title":"Promega, JA9011, TLR Reporter Bioassay","description":"\u003cp\u003eMeasure Potency and Stability of Molecules that Modulate TLR Signaling\u003cbr\u003e\n96- and 384-well plate formats\u003cbr\u003e\nNo primary cell culture required\u003cbr\u003e\nSupplied in convenient thaw-and-use format; also available in cell propagation model (CPM) format\u003cbr\u003e\nSpecifications：\u003cbr\u003e\nTLR Bioassay Cells\tGA132A\t1 × 0.5ml\u003cbr\u003e\nFetal Bovine Serum\tJ121A\t1 × 4ml\u003cbr\u003e\nRPMI 1640 Medium\tG708A\t1 × 36ml\u003cbr\u003e\nBio-Glo-NL™ Luciferase Assay Buffer\tJ308A\t1 × 10ml\u003cbr\u003e\nBio-Glo-NL™ Luciferase Assay Substrate\tJ309A\t1 × 200μl\u003cbr\u003e\nTLR Bioassay Cells\tGA132A\t5 × 0.5ml\u003cbr\u003e\nFetal Bovine Serum\tJ121A\t5 × 4ml\u003cbr\u003e\nRPMI 1640 Medium\tG708A\t5 × 36ml\u003cbr\u003e\nBio-Glo-NL™ Luciferase Assay Buffer\tJ308A\t5 × 10ml\u003cbr\u003e\nBio-Glo-NL™ Luciferase Assay Substrate\tJ309A\t5 × 200μl\u003cbr\u003e\nTLR Bioassay Cells (CPM)\tGA130A\t2 × 1ml\u003cbr\u003e\nTLR Bioassay Cells (CPM)\tGA130A\t50 × 1ml\u003cbr\u003e\nThe human immune system responds to conserved damage- and pathogen-associated molecular patterns (DAMPs and PAMPs, respectively) via pattern-recognition receptors (PRRs). One such family of PRRs is the Toll-like receptor (TLR) family. In humans, there are 10 known TLRs, which enable the immune system to sense and respond to damage and danger signals. Modulation of the TLR system, either by agonism or antagonism, has the potential to alter immune and inflammatory responses. Blockade of TLRs may inhibit inflammatory responses while TLR agonists may promote immune responses against pathogens or cancer.\u003cbr\u003e\nCurrent methods for assessing the activity of TLR modulators typically rely on primary monocyte-derived macrophages and measurement of functional endpoints, such as cytokine production. These assays are laborious and highly variable due to their reliance on donor cells, complex assay protocols and unqualified assay reagents. As a result, these assays are difficult to establish in a quality-controlled setting.\u003cbr\u003e\nThe TLR Bioassay is a bioluminescent reporter cell-based assay used to measure the potency and stability of TLR agonists.\u003c\/p\u003e","brand":"Promega","offers":[{"title":"Default Title","offer_id":46835631030441,"sku":"JA9011","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/promega-ja9011","provider":"Iright","version":"1.0","type":"link"}