{"product_id":"promega-ja9411","title":"Promega, JA9411, ADCP Reporter Bioassay (THP-1)","description":"\u003cp\u003eMeasure Potency and Stability of Biologics that Bind and Activate ADCP through FcγRs\u003cbr\u003e\nPrequalified according to ICH guidelines\u003cbr\u003e\n96- and 384-well plate formats\u003cbr\u003e\nNo primary cell culture required\u003cbr\u003e\nSupplied in convenient thaw-and-use format; also available in cell propagation model (CPM) format\u003cbr\u003e\nSpecifications：\u003cbr\u003e\nADCP Bioassay Effector Cells (THP-1)\tGA128A\t1 × 0.5ml\u003cbr\u003e\nRPMI 1640 Medium\tG708A\t1 × 36ml\u003cbr\u003e\nLow IgG Serum\tG711A\t1 × 4ml\u003cbr\u003e\nBio-Glo-NL™ Luciferase Assay Buffer\tJ308A\t1 × 10ml\u003cbr\u003e\nBio-Glo-NL™ Luciferase Assay Substrate\tJ309A\t1 × 200μl\u003cbr\u003e\nADCP Bioassay Effector Cells (THP-1)\tGA128A\t5 × 0.5ml\u003cbr\u003e\nRPMI 1640 Medium\tG708A\t5 × 36ml\u003cbr\u003e\nLow IgG Serum\tG711A\t5 × 4ml\u003cbr\u003e\nBio-Glo-NL™ Luciferase Assay Buffer\tJ308A\t5 × 10ml\u003cbr\u003e\nBio-Glo-NL™ Luciferase Assay Substrate\tJ309A\t5 × 200μl\u003cbr\u003e\nADCP Bioassay Effector Cells (THP-1) (CPM)\tGA127A\t2 × 1ml\u003cbr\u003e\nADCP Bioassay Effector Cells (THP-1) (CPM)\tGA127A\t50 × 1ml\u003cbr\u003e\nAntibody-dependent cellular phagocytosis (ADCP) is an important mechanism-of-action (MOA) for antibody-based immunotherapies. ADCP can be mediated by monocytes, macrophages, neutrophils and dendritic cells via FcγRIIa, FcγRI and FcγRIIIa. In myeloid cells, the expression level of the various receptors is highly dynamic and influenced by cell lineage, tissue microenvironment and local inflammatory state. These receptors can participate in antibody recognition, receptor clustering and signaling events that result in ADCP.\u003cbr\u003e\nCurrent methods for measuring ADCP rely on the isolation of primary human monocytes, ex vivo differentiation into macrophages and direct measurement of phagocytosis. These assays are laborious and highly variable due to their reliance on donor cells, complex assay protocols and unqualified assay reagents. As a result, these assays are difficult to establish in quality-controlled, drug development settings.\u003cbr\u003e\nThe ADCP Reporter Bioassay (THP-1) is a bioluminescent reporter cell-based assay used to measure the potency and stability of antibodies and other biologics with Fc domains that bind and activate FcγRs.\u003c\/p\u003e","brand":"Promega","offers":[{"title":"Default Title","offer_id":46835622903977,"sku":"JA9411","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/promega-ja9411","provider":"Iright","version":"1.0","type":"link"}