{"product_id":"promega-k1210","title":"Promega, K1210, TIM-3 Bioassay","description":"\u003cp\u003eMeasure Potency and Stability of Biologics Designed to Block TIM-3 Interactions\u003cbr\u003e\nUses Bio-Glo-NL™ Luciferase Assay System\u003cbr\u003e\n96- and 384-well plate formats\u003cbr\u003e\nNo primary cell culture required\u003cbr\u003e\nSupplied in convenient thaw-and-use format and cell propagation model (CPM) format\u003cbr\u003e\nSpecifications：\u003cbr\u003e\nTIM-3 Effector Cells\tJ313A\t1 × 0.5ml\u003cbr\u003e\nTIM-3 Target Cells\tJ314A\t1 × 0.9ml\u003cbr\u003e\nFetal Bovine Serum\tJ121A\t1 × 4ml\u003cbr\u003e\nRPMI 1640 Medium\tG708A\t1 × 36ml\u003cbr\u003e\nBio-Glo-NL™ Luciferase Assay Buffer\tJ308A\t1 × 10ml\u003cbr\u003e\nBio-Glo-NL™ Luciferase Assay Substrate\tJ309A\t1 × 200μl\u003cbr\u003e\nTIM-3 Effector Cells\tJ313A\t5 × 0.5ml\u003cbr\u003e\nTIM-3 Target Cells\tJ314A\t5 × 0.9ml\u003cbr\u003e\nFetal Bovine Serum\tJ121A\t5 × 4ml\u003cbr\u003e\nRPMI 1640 Medium\tG708A\t5 × 36ml\u003cbr\u003e\nBio-Glo-NL™ Luciferase Assay Buffer\tJ308A\t5 × 10ml\u003cbr\u003e\nBio-Glo-NL™ Luciferase Assay Substrate\tJ309A\t5 × 200μl\u003cbr\u003e\nTIM-3 Effector Cells (CPM)\tJ306A\t2 × 1ml\u003cbr\u003e\nTIM-3 Target Cells (CPM)\tJ307A\t2 × 1ml\u003cbr\u003e\nControl Ab, Anti-TIM-3\tK121A\t1 × 100μg\u003cbr\u003e\nThe TIM-3 Bioassay is a bioluminescent reporter cell-based assay that overcomes the limitations of existing assays and can be used to measure the potency and stability of antibodies and other biologics targeting TIM-3. TIM-3 (CD366, HAVCR2) is an immune checkpoint receptor expressed on a subset of activated T cells, regulatory T cells (Tregs), macrophages and dendritic cells. TIM-3 has the capacity to inhibit or costimulate T-cell receptor (TCR) signaling in different in vitro systems.\u003cbr\u003e\nThe TIM-3 Bioassay consists of two cell lines:\u003cbr\u003e\nWhen the two cell types are co-cultured, TIM-3 Target Cells provide low-level stimulation to the TIM-3 Effector Cells through the TCR. The combined stimulation of the TCR and TIM-3 induces promoter-mediated luciferase activation and luminescence. Adding a TIM-3 blocking antibody prevents TIM-3 signaling and reduces promoter-mediated luminescence.\u003cbr\u003e\nThe Control Ab, Anti-TIM-3, is a mouse anti-human TIM-3 antibody that shows blocking activity for TIM-3 with its ligands. It is intended for use as a control with the TIM-3 Bioassay, which can be used to measure the potency and stability of antibodies and other biologics designed to block the TIM-3 signaling.\u003c\/p\u003e","brand":"Promega","offers":[{"title":"Default Title","offer_id":46835629523113,"sku":"K1210","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/promega-k1210","provider":"Iright","version":"1.0","type":"link"}