{"product_id":"promega-n1663","title":"Promega, N1663, NanoBRET® Nano-Glo® Detection Systems","description":"\u003cp\u003eDedicated Detection Solutions for NanoBRET® Protein Interaction Assays\u003cbr\u003e\nMeasure protein interactions in live cells with full-length proteins\u003cbr\u003e\nUse the standard reagent for single-time-point or \u0026lt;2-hour analysis; use the kinetic reagent for extended live-cell measurements\u003cbr\u003e\nIncreased signal and lower background compared to conventional BRET assays\u003cbr\u003e\nSpecifications：\u003cbr\u003e\nHaloTag® NanoBRET® 618 Ligand\tG980A\t1 × 20μl\u003cbr\u003e\nNanoBRET® Nano-Glo® Substrate\tN157A\t1 × 50μl\u003cbr\u003e\nHaloTag® NanoBRET® 618 Ligand\tG980A\t5 × 20μl\u003cbr\u003e\nNanoBRET® Nano-Glo® Substrate\tN157A\t5 × 50μl\u003cbr\u003e\nHaloTag® NanoBRET® 618 Ligand\tG980B\t1 × 1ml\u003cbr\u003e\nNanoBRET® Nano-Glo® Substrate\tN157B\t2 × 1.25ml\u003cbr\u003e\nHaloTag® NanoBRET® 618 Ligand\tG980A\t1 × 20μl\u003cbr\u003e\nNano-Glo® Vivazine® Substrate, 100X\tN258A\t2 × 0.1ml\u003cbr\u003e\nHaloTag® NanoBRET® 618 Ligand\tG980A\t5 × 20μl\u003cbr\u003e\nNano-Glo® Vivazine® Substrate, 100X\tN258B\t1 × 1ml\u003cbr\u003e\nHaloTag® NanoBRET® 618 Ligand\tG980B\t1 × 1ml\u003cbr\u003e\nNano-Glo® Vivazine® Substrate, 100X\tN258B\t10 × 1ml\u003cbr\u003e\nThe NanoBRET® assay is a bioluminescence resonance energy transfer (BRET)-based assay that uses NanoLuc® Luciferase as the BRET energy donor and HaloTag® protein labeled with the HaloTag® NanoBRET® 618 fluorescent Ligand as the energy acceptor to measure the interaction of two binding partners in live cells.\u003cbr\u003e\nThe NanoBRET® Nano-Glo® Detection System provides the NanoBRET® Nano-Glo® Substrate used by NanoLuc® Luciferase to generate the donor signal and the HaloTag® NanoBRET® 618 Ligand for the fluorescent energy acceptor. The HaloTag® NanoBRET® 618 Ligand is added directly to the cells during plating, and the NanoBRET® Nano-Glo® Substrate is added to the sample just prior to measuring donor and acceptor emission. The NanoBRET® Nano-Glo® Kinetic Detection System has the Vivazine® substrate for use by the NanoLuc® luciferase donor, giving an extended signal for live-cell kinetic assays.\u003cbr\u003e\nFor more details on using the NanoBRET® assay for protein:protein interaction studies visit:\u003cbr\u003e\nNanoBRET® Technology for Protein Interactions\u003cbr\u003e\n. The NanoBRET® Nano-Glo® Detection Systems are compatible with a diverse set of prebuilt or custom NanoBRET® PPI Assays.\u003cbr\u003e\nNote:\u003cbr\u003e\nThe total number of assays will vary based on final well volume. For standard well volumes:\u003cbr\u003e\nCat.# N1661 and N2583 are sufficient for 200 assays in 96-well plates or 500 assays in 384-well plates.\u003cbr\u003e\nCat.# N1662 and N2584 are sufficient for 1,000 assays in 96-well plates or 2,500 assays in 384-well plates.\u003cbr\u003e\nCat.# N1663 and N2585 are sufficient for 10,000 assays in 96-well plates or 25,000 assays in 384-well plates.\u003c\/p\u003e","brand":"Promega","offers":[{"title":"Default Title","offer_id":46835699679401,"sku":"N1663","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/promega-n1663","provider":"Iright","version":"1.0","type":"link"}